Detailed information of TA system TA031892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 603623..604665
• Replicon: chromosome
• Accession: NZ_AP018560
• Organism: Aerosticca soli strain Dysh456

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: ALSL_RS02695
• Protein ID: WP_003109777.1
• Coordinates: 604090..604665 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: ALSL_RS02690
• Protein ID: WP_003050245.1
• Coordinates: 603623..604093 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALSL_RS02655	599015..600433	-	1419	WP_020924934.1	TIGR03752 family integrating conjugative element protein	-
ALSL_RS02660	600423..601334	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
ALSL_RS02665	601331..602023	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
ALSL_RS02670	602020..602418	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
ALSL_RS02675	602430..602789	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
ALSL_RS02680	602806..603039	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
ALSL_RS02685	603036..603419	-	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
ALSL_RS02690	603623..604093	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
ALSL_RS02695	604090..604665	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
ALSL_RS02700	604683..605597	+	915	WP_003105629.1	AAA family ATPase	-
ALSL_RS02705	605594..606064	+	471	WP_003105626.1	hypothetical protein	-
ALSL_RS02710	606061..606561	+	501	WP_003090159.1	hypothetical protein	-
ALSL_RS02715	606561..607463	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
ALSL_RS02720	607502..608227	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	514541..656523	141982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T31892 WP_003109777.1 NZ_AP018560:604090-604665 [Aerosticca soli]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T31892 NZ_AP018560:604090-604665 [Aerosticca soli]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT31892 WP_003050245.1 NZ_AP018560:603623-604093 [Aerosticca soli]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT31892 NZ_AP018560:603623-604093 [Aerosticca soli]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68