Detailed information of TA system TA031889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 8028..8572
• Replicon: plasmid pTH1
• Accession: NZ_AP018559
• Organism: Hydrogenophilus thermoluteolus strain TH-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HPTL_RS10845
• Protein ID: WP_179949102.1
• Coordinates: 8028..8354 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HPTL_RS10850
• Protein ID: WP_119336199.1
• Coordinates: 8351..8572 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPTL_RS11225	3611..3850	+	240	WP_145981821.1	hypothetical protein	-
HPTL_RS10820	3980..4306	-	327	WP_119336194.1	type II toxin-antitoxin system PemK/MazF family toxin	-
HPTL_RS10825	4293..4523	-	231	WP_119336195.1	addiction module antitoxin	-
HPTL_RS10830	4597..6261	-	1665	WP_119336196.1	relaxase/mobilization nuclease domain-containing protein	-
HPTL_RS10835	6251..6634	-	384	WP_119336197.1	plasmid mobilization relaxosome protein MobC	-
HPTL_RS11310	7519..7662	+	144	Protein_10	ATP-binding protein	-
HPTL_RS10840	7670..7921	-	252	WP_119336198.1	hypothetical protein	-
HPTL_RS10845	8028..8354	-	327	WP_179949102.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HPTL_RS10850	8351..8572	-	222	WP_119336199.1	antitoxin MazE family protein	Antitoxin
HPTL_RS10855	8729..10003	-	1275	WP_119336200.1	transposase	-
HPTL_RS10860	9997..10626	-	630	WP_119336201.1	IS607 family transposase	-
HPTL_RS10865	10829..11242	+	414	WP_119336202.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPTL_RS10870	11196..11516	+	321	WP_119336203.1	DNA-binding transcriptional regulator	-
HPTL_RS11340	11629..12159	-	531	WP_179949103.1	hypothetical protein	-
HPTL_RS10885	12603..12770	+	168	WP_179949104.1	hypothetical protein	-
HPTL_RS10890	12812..13048	+	237	WP_119336204.1	ribbon-helix-helix protein, CopG family	-
HPTL_RS10895	13045..13476	+	432	WP_119336205.1	putative toxin-antitoxin system toxin component, PIN family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ureB / ureA	1..65637	65637

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (14-105)

Antitoxin

• Antitoxin MazE-like (7-71)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11893.00 Da
• Isoelectric Point: 9.2935

>T31889 WP_179949102.1 NZ_AP018559:c8354-8028 [Hydrogenophilus thermoluteolus]
MMRGDLVTIVTQGDFGKPRPALVIQSDLFNELSSITVFPVTSTIVDAPLLRVTVEPTPENGLHKRSQVMVDKIMTVRRDK
VGQIIGRIDTTTLTTIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T31889 NZ_AP018559:c8354-8028 [Hydrogenophilus thermoluteolus]
ATGATGCGCGGCGATTTGGTAACGATCGTTACCCAGGGCGACTTTGGCAAACCAAGGCCAGCGTTGGTGATCCAGTCCGA
TCTGTTCAACGAGCTCAGCAGCATAACCGTCTTTCCTGTAACCAGTACCATTGTGGATGCCCCGTTGTTGCGCGTCACGG
TTGAGCCAACCCCGGAAAACGGCTTGCACAAGCGATCACAGGTGATGGTGGACAAAATTATGACCGTGCGGCGGGACAAA
GTCGGTCAGATCATTGGACGTATCGATACGACTACGCTGACAACGATCGAGCGCTGCTTAGCTGTCTTCTTGGGAATCGC
GAAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8394.66 Da
• Isoelectric Point: 9.3851

>AT31889 WP_119336199.1 NZ_AP018559:c8572-8351 [Hydrogenophilus thermoluteolus]
MTVPVKARVQKYRDALRRSGLRPVQIWVPDTRRPGFAQECRRQCLLVTQADAADTQLQRLMDEALAEIDGWQA

Nucleotide

• Length: 222 bp

>AT31889 NZ_AP018559:c8572-8351 [Hydrogenophilus thermoluteolus]
ATGACAGTACCGGTCAAAGCGCGTGTTCAAAAGTATCGCGACGCGCTGCGCCGCTCGGGTTTGCGTCCGGTTCAGATCTG
GGTACCGGATACCCGCCGCCCGGGCTTTGCACAAGAGTGTCGCCGCCAGTGCCTGCTCGTGACCCAGGCAGACGCCGCTG
ATACGCAACTCCAACGACTCATGGATGAAGCCTTGGCAGAGATTGATGGCTGGCAGGCATGA