Detailed information of TA system TA031868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 1780995..1781688
• Replicon: chromosome
• Accession: NZ_AP018543
• Organism: Enterococcus faecalis strain KUB3007

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A2Z6BTE2
• Locus tag: EL279_RS09110
• Protein ID: WP_002364356.1
• Coordinates: 1781344..1781688 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: EL279_RS09105
• Protein ID: WP_002364355.1
• Coordinates: 1780995..1781327 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL279_RS09060	1776712..1777467	-	756	WP_002391449.1	DnaD domain protein	-
EL279_RS09065	1777482..1778297	-	816	WP_010783770.1	PD-(D/E)XK nuclease-like domain-containing protein	-
EL279_RS09070	1778260..1779276	-	1017	WP_010783771.1	recombinase RecT	-
EL279_RS09080	1779379..1779603	-	225	WP_002391454.1	hypothetical protein	-
EL279_RS09085	1779600..1779923	-	324	WP_010783772.1	hypothetical protein	-
EL279_RS09090	1779963..1780178	-	216	WP_010783773.1	hypothetical protein	-
EL279_RS09095	1780185..1780496	-	312	WP_002381719.1	hypothetical protein	-
EL279_RS09100	1780507..1780683	-	177	WP_002364354.1	helix-turn-helix transcriptional regulator	-
EL279_RS09105	1780995..1781327	+	333	WP_002364355.1	helix-turn-helix domain-containing protein	Antitoxin
EL279_RS09110	1781344..1781688	+	345	WP_002364356.1	ImmA/IrrE family metallo-endopeptidase	Toxin
EL279_RS09115	1781782..1782687	+	906	WP_016630909.1	DUF4352 domain-containing protein	-
EL279_RS09125	1782855..1783583	+	729	WP_002364358.1	ion transporter	-
EL279_RS09130	1783679..1784827	+	1149	WP_010783774.1	site-specific integrase	-
EL279_RS09135	1784855..1785298	-	444	WP_033786344.1	cleavage protein	-
EL279_RS09140	1785295..1785570	-	276	WP_002356991.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
EL279_RS09145	1785570..1786616	-	1047	WP_033786346.1	type II secretion system F family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (22-81)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13704.62 Da
• Isoelectric Point: 5.8535

>T31868 WP_002364356.1 NZ_AP018543:1781344-1781688 [Enterococcus faecalis]
MKSIKELVEEYEVELVFAPINKRACYEPVKRIIFVNQNLSIEEQEESIFHEFKHVVSHSDYMELYKIPSFRNKMEAEADH
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLN

Nucleotide

• Length: 345 bp

>T31868 NZ_AP018543:1781344-1781688 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAGCGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAGTCTATATTCCATGAGTTCAAAC
ATGTTGTTTCCCATTCGGATTACATGGAGTTATATAAAATCCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCGGATCAT
CATATGTTTAAATGCCTTATTGAAAAACATGACGGGCAATTTAATTATTCTAATGTGATTACTCATTACAATTTAAAGAT
GGGACAAGAAACTTATTTAAATTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12691.16 Da
• Isoelectric Point: 4.6307

>AT31868 WP_002364355.1 NZ_AP018543:1780995-1781327 [Enterococcus faecalis]
MNLLERIKKLAKDRDVSIYQLEEEINIGRNTIYQWNKRTPSSDKLEAVANYFNVSVDYLLGRTENPNQAGAKPSDDLDEV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT31868 NZ_AP018543:1780995-1781327 [Enterococcus faecalis]
ATGAACCTCTTGGAACGTATAAAAAAACTAGCAAAAGATAGAGATGTGTCTATTTATCAGCTTGAAGAAGAAATAAATAT
TGGACGAAATACTATTTATCAATGGAATAAGCGAACACCTTCTTCGGATAAACTTGAAGCTGTAGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTGGTGCTAAACCATCTGATGATTTAGATGAAGTA
TTAGACAATGTGATGAGTTTCGATGGTGAGCCTTTGGATGATCATGATCGGGAAGTTATTCGTGCTTATTTGAAAGGGAG
ATTCGGAAAATAA

Structures

Toxin

• AlphaFold DB: A0A2Z6BTE2