Detailed information of TA system TA031864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 70119..70738
• Replicon: plasmid pKUB3006-2
• Accession: NZ_AP018540
• Organism: Enterococcus faecalis strain KUB3006

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: EL240_RS15570
• Protein ID: WP_000241511.1
• Coordinates: 70119..70481 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3H5D1
• Locus tag: EL240_RS15575
• Protein ID: WP_000245205.1
• Coordinates: 70475..70738 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL240_RS15555	65529..67460	-	1932	WP_010816308.1	sucrose-specific PTS transporter subunit IIBC	-
EL240_RS15560	67651..69090	+	1440	WP_002367771.1	sucrose-6-phosphate hydrolase	-
EL240_RS15565	69092..70054	+	963	WP_002367770.1	LacI family DNA-binding transcriptional regulator	-
EL240_RS15570	70119..70481	-	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EL240_RS15575	70475..70738	-	264	WP_000245205.1	PbsX family transcriptional regulator	Antitoxin
EL240_RS15580	70915..71535	+	621	WP_161971465.1	recombinase family protein	-
EL240_RS15585	71552..71839	+	288	WP_080114361.1	hypothetical protein	-
EL240_RS15590	71833..72048	+	216	WP_010717965.1	hypothetical protein	-
EL240_RS15600	72651..72859	+	209	Protein_65	DNA helicase UvrB	-
EL240_RS15605	72912..73277	+	366	WP_002409263.1	SH3 domain-containing protein	-
EL240_RS15615	73614..73865	+	252	WP_002358196.1	hypothetical protein	-
EL240_RS15620	73981..75297	+	1317	WP_010717968.1	Y-family DNA polymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	prgB/asc10	1..79566	79566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T31864 WP_000241511.1 NZ_AP018540:c70481-70119 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T31864 NZ_AP018540:c70481-70119 [Enterococcus faecalis]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10059.46 Da
• Isoelectric Point: 5.1585

>AT31864 WP_000245205.1 NZ_AP018540:c70738-70475 [Enterococcus faecalis]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Nucleotide

• Length: 264 bp

>AT31864 NZ_AP018540:c70738-70475 [Enterococcus faecalis]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTGCATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Structures

Toxin

• AlphaFold DB: R3H4V2

Antitoxin

• AlphaFold DB: R3H5D1