Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 51579..52150 Replicon plasmid pKUB3006-2
Accession NZ_AP018540
Organism Enterococcus faecalis strain KUB3006

Toxin (Protein)


Gene name mazF Uniprot ID A0A2P6BPI5
Locus tag EL240_RS15490 Protein ID WP_002394791.1
Coordinates 51809..52150 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag EL240_RS15485 Protein ID WP_002362431.1
Coordinates 51579..51809 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EL240_RS15455 46758..47021 - 264 WP_010817818.1 hypothetical protein -
EL240_RS15460 47170..47637 + 468 WP_010817817.1 single-stranded DNA-binding protein -
EL240_RS15465 47732..48289 + 558 WP_126301007.1 thermonuclease family protein -
EL240_RS15470 48562..49149 + 588 WP_057086840.1 hypothetical protein -
EL240_RS15475 49351..50559 + 1209 WP_126301008.1 hypothetical protein -
EL240_RS15480 50570..51364 + 795 WP_057086842.1 hypothetical protein -
EL240_RS15485 51579..51809 + 231 WP_002362431.1 hypothetical protein Antitoxin
EL240_RS15490 51809..52150 + 342 WP_002394791.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EL240_RS15495 52262..53200 + 939 WP_002394789.1 hypothetical protein -
EL240_RS15500 53468..54070 + 603 WP_002367780.1 Fic family protein -
EL240_RS15505 54304..54984 - 681 WP_002367779.1 IS6 family transposase -
EL240_RS15510 55239..56177 + 939 WP_002335374.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - prgB/asc10 1..79566 79566


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13248.49 Da        Isoelectric Point: 8.8595

>T31863 WP_002394791.1 NZ_AP018540:51809-52150 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T31863 NZ_AP018540:51809-52150 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGTTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT31863 WP_002362431.1 NZ_AP018540:51579-51809 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp

>AT31863 NZ_AP018540:51579-51809 [Enterococcus faecalis]
ATGTTAACAACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P6BPI5

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References