Detailed information of TA system TA031862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 83529..84637
• Replicon: plasmid pKUB3006-1
• Accession: NZ_AP018539
• Organism: Enterococcus faecalis strain KUB3006

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: EL240_RS15165
• Protein ID: WP_071661597.1
• Coordinates: 83768..84637 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: EL240_RS15160
• Protein ID: WP_000205227.1
• Coordinates: 83529..83753 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL240_RS15135	79160..80644	+	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-
EL240_RS15140	80698..81501	+	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
EL240_RS15150	81810..83111	-	1302	Protein_93	ISL3 family transposase	-
EL240_RS15155	83252..83386	+	135	Protein_94	site-specific recombinase	-
EL240_RS15160	83529..83753	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
EL240_RS15165	83768..84637	+	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
EL240_RS15170	84618..85352	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
EL240_RS16115	85385..85501	+	117	Protein_98	aminoglycoside 6-adenylyltransferase	-
EL240_RS15175	85514..85906	+	393	WP_000393259.1	GNAT family N-acetyltransferase	-
EL240_RS15180	85907..86432	+	526	Protein_100	AAC(6')-Ie family aminoglycoside N-acetyltransferase	-
EL240_RS15185	86463..87338	+	876	WP_063610281.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
EL240_RS15190	87626..88804	+	1179	WP_000997695.1	IS256-like element ISEf1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / aph(3')-III / dfrG / ant(6)-Ia / lsa(E) / lnu(B) / aac(6')-aph(2'')	prgB/asc10	1..106370	106370
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III / erm(B) / lsa(E) / lnu(B) / aac(6')-aph(2'')	-	66133..88804	22671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T31862 WP_071661597.1 NZ_AP018539:83768-84637 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T31862 NZ_AP018539:83768-84637 [Enterococcus faecalis]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGTATCAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAACATAAACCTGAAAAATATACCGAG
AAGGTAAATCATATTTTTGAAGTACTCGGTATCTCACTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT31862 WP_000205227.1 NZ_AP018539:83529-83753 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT31862 NZ_AP018539:83529-83753 [Enterococcus faecalis]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6