Detailed information of TA system TA031861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 60229..61366
• Replicon: plasmid pKUB3006-1
• Accession: NZ_AP018539
• Organism: Enterococcus faecalis strain KUB3006

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EL240_RS15015
• Protein ID: WP_002333003.1
• Coordinates: 60503..61366 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: B3CKC7
• Locus tag: EL240_RS15010
• Protein ID: WP_002333002.1
• Coordinates: 60229..60501 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EL240_RS14990	56556..56792	-	237	Protein_63	hypothetical protein	-
EL240_RS14995	57067..58905	+	1839	WP_104681177.1	type IA DNA topoisomerase	-
EL240_RS15000	59008..59904	+	897	WP_104681373.1	ParA family protein	-
EL240_RS15005	60002..60211	+	210	WP_000527318.1	peptide-binding protein	-
EL240_RS15010	60229..60501	+	273	WP_002333002.1	antitoxin	Antitoxin
EL240_RS15015	60503..61366	+	864	WP_002333003.1	zeta toxin family protein	Toxin
EL240_RS15020	61488..61985	+	498	Protein_69	DnaJ domain-containing protein	-
EL240_RS15025	62070..62666	-	597	WP_085442989.1	TetR/AcrR family transcriptional regulator	-
EL240_RS15030	62977..63858	+	882	WP_002326774.1	ABC transporter ATP-binding protein	-
EL240_RS15035	63882..65495	+	1614	WP_002333004.1	hypothetical protein	-
EL240_RS15040	65567..66091	-	525	WP_010717848.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / aph(3')-III / dfrG / ant(6)-Ia / lsa(E) / lnu(B) / aac(6')-aph(2'')	prgB/asc10	1..106370	106370
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III / erm(B) / lsa(E) / lnu(B) / aac(6')-aph(2'')	-	66133..88804	22671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32365.76 Da
• Isoelectric Point: 6.3643

>T31861 WP_002333003.1 NZ_AP018539:60503-61366 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVVVIDNDTFKQQHPNFDELV
KLYEKDVVKHATPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKTYAMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T31861 NZ_AP018539:60503-61366 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAGTTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGAAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTGTTGTCATTGATAATGATACCTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACACCTTATTCTAATCGTATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGCACAGGACGAACAACAGACGTTCCTATTCAAACAGCCACAATGCTACAAG
CCAAAGGTTATGAAACAAAAACGTACGCCATGGCAGTACCCAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.29 Da
• Isoelectric Point: 7.1987

>AT31861 WP_002333002.1 NZ_AP018539:60229-60501 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELKAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT31861 NZ_AP018539:60229-60501 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTAAAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2Z6BWJ3