31827

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4457063..4457694 Replicon chromosome
Accession NZ_AP018488
Organism Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag ECpv15279_RS23275 Protein ID WP_001259385.1
Coordinates 4457419..4457694 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ECpv15279_RS23270 Protein ID WP_000593555.1
Coordinates 4457063..4457422 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECpv15279_RS23240 4452175..4452648 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
ECpv15279_RS23245 4452645..4452926 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ECpv15279_RS23250 4453003..4454361 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
ECpv15279_RS23255 4454354..4455862 + 1509 WP_001301773.1 carbohydrate kinase -
ECpv15279_RS23260 4455852..4456121 + 270 WP_000933060.1 HPr family phosphocarrier protein -
ECpv15279_RS23265 4456153..4457013 + 861 WP_001131788.1 class II aldolase -
ECpv15279_RS23270 4457063..4457422 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECpv15279_RS23275 4457419..4457694 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
ECpv15279_RS23280 4457767..4458891 - 1125 WP_001301659.1 ABC transporter permease -
ECpv15279_RS23285 4458891..4461626 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
ECpv15279_RS23290 4461623..4462690 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T31827 WP_001259385.1 NZ_AP018488:c4457694-4457419 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T31827 NZ_AP018488:c4457694-4457419 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT31827 WP_000593555.1 NZ_AP018488:c4457422-4457063 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT31827 NZ_AP018488:c4457422-4457063 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References