Detailed information of TA system TA031821

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3881831..3882485
• Replicon: chromosome
• Accession: NZ_AP018488
• Organism: Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1PAM6
• Locus tag: ECpv15279_RS20245
• Protein ID: WP_000244781.1
• Coordinates: 3881831..3882238 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: ECpv15279_RS20250
• Protein ID: WP_000354048.1
• Coordinates: 3882219..3882485 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECpv15279_RS20225	3877788..3879521	-	1734	WP_000813185.1	single-stranded-DNA-specific exonuclease RecJ	-
ECpv15279_RS20230	3879527..3880237	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
ECpv15279_RS20235	3880262..3881158	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
ECpv15279_RS20240	3881270..3881791	+	522	WP_001055874.1	flavodoxin FldB	-
ECpv15279_RS20245	3881831..3882238	-	408	WP_000244781.1	protein YgfX	Toxin
ECpv15279_RS20250	3882219..3882485	-	267	WP_000354048.1	FAD assembly factor SdhE	Antitoxin
ECpv15279_RS20255	3882728..3883708	+	981	WP_000886053.1	tRNA-modifying protein YgfZ	-
ECpv15279_RS20260	3883785..3884444	-	660	WP_000250274.1	hemolysin III family protein	-
ECpv15279_RS20265	3884608..3884919	-	312	WP_001182957.1	N(4)-acetylcytidine aminohydrolase	-
ECpv15279_RS20270	3884964..3886397	+	1434	WP_001310226.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16061.99 Da
• Isoelectric Point: 11.5202

>T31821 WP_000244781.1 NZ_AP018488:c3882238-3881831 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T31821 NZ_AP018488:c3882238-3881831 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10561.15 Da
• Isoelectric Point: 5.1700

>AT31821 WP_000354048.1 NZ_AP018488:c3882485-3882219 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPELFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT31821 NZ_AP018488:c3882485-3882219 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGAGC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1PAM6