Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1368070..1368865 Replicon chromosome
Accession NZ_AP018488
Organism Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag ECpv15279_RS06650 Protein ID WP_000854914.1
Coordinates 1368491..1368865 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag ECpv15279_RS06645 Protein ID WP_001280954.1
Coordinates 1368070..1368444 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECpv15279_RS06610 1363425..1364330 + 906 WP_000203541.1 chemotaxis protein -
ECpv15279_RS06615 1364327..1365397 + 1071 WP_000102669.1 patatin-like phospholipase family protein -
ECpv15279_RS06625 1365737..1366555 + 819 WP_001234682.1 DUF945 domain-containing protein -
ECpv15279_RS06630 1366646..1367131 + 486 WP_000214388.1 antirestriction protein -
ECpv15279_RS06635 1367147..1367623 + 477 WP_001475628.1 RadC family protein -
ECpv15279_RS06640 1367686..1367907 + 222 WP_001220314.1 DUF987 domain-containing protein -
ECpv15279_RS06645 1368070..1368444 + 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ECpv15279_RS06650 1368491..1368865 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
ECpv15279_RS06655 1368862..1369353 + 492 WP_000976857.1 hypothetical protein -
ECpv15279_RS06660 1369365..1369562 + 198 WP_000839282.1 DUF957 domain-containing protein -
ECpv15279_RS06665 1369647..1370489 + 843 WP_001280481.1 DUF4942 domain-containing protein -
ECpv15279_RS06675 1370960..1371898 + 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
ECpv15279_RS06680 1371953..1372690 + 738 WP_000283664.1 zinc-binding phosphatase -
ECpv15279_RS06685 1372714..1373268 + 555 WP_001001917.1 molecular chaperone YcdY -
ECpv15279_RS06690 1373370..1373861 + 492 WP_001301587.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T31802 WP_000854914.1 NZ_AP018488:1368491-1368865 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T31802 NZ_AP018488:1368491-1368865 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT31802 WP_001280954.1 NZ_AP018488:1368070-1368444 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT31802 NZ_AP018488:1368070-1368444 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References