Detailed information of TA system TA031799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1153967..1154445
• Replicon: chromosome
• Accession: NZ_AP018488
• Organism: Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: ECpv15279_RS05435
• Protein ID: WP_001303876.1
• Coordinates: 1153967..1154254 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: ECpv15279_RS05440
• Protein ID: WP_000536233.1
• Coordinates: 1154254..1154445 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECpv15279_RS05400	1149117..1151588	-	2472	WP_000034474.1	exonuclease	-
ECpv15279_RS05405	1151682..1151873	-	192	WP_001098307.1	DUF1482 family protein	-
ECpv15279_RS05410	1151870..1152058	-	189	WP_000413706.1	hypothetical protein	-
ECpv15279_RS05420	1152632..1152817	+	186	WP_001133046.1	hypothetical protein	-
ECpv15279_RS05425	1153004..1153393	-	390	WP_000394511.1	hypothetical protein	-
ECpv15279_RS05430	1153535..1153690	-	156	WP_000379575.1	DUF1391 family protein	-
ECpv15279_RS05435	1153967..1154254	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECpv15279_RS05440	1154254..1154445	-	192	WP_000536233.1	hypothetical protein	Antitoxin
ECpv15279_RS05445	1154473..1154874	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
ECpv15279_RS05450	1154983..1155255	+	273	WP_000887446.1	helix-turn-helix domain-containing protein	-
ECpv15279_RS05455	1155239..1155664	+	426	WP_000693855.1	Rha family transcriptional regulator	-
ECpv15279_RS05460	1155871..1156326	-	456	WP_000273724.1	hypothetical protein	-
ECpv15279_RS05465	1156405..1157496	+	1092	WP_001205823.1	hypothetical protein	-
ECpv15279_RS05470	1157503..1158249	+	747	WP_000788751.1	ATP-binding protein	-
ECpv15279_RS05475	1158271..1159041	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
ECpv15279_RS05480	1159057..1159293	+	237	Protein_1047	DUF977 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T31799 WP_001303876.1 NZ_AP018488:c1154254-1153967 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T31799 NZ_AP018488:c1154254-1153967 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT31799 WP_000536233.1 NZ_AP018488:c1154445-1154254 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT31799 NZ_AP018488:c1154445-1154254 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA