31794

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 54796..55346 Replicon chromosome
Accession NZ_AP018488
Organism Escherichia coli O157:H7 strain PV15-279

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag ECpv15279_RS00250 Protein ID WP_001160975.1
Coordinates 55032..55346 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag ECpv15279_RS00245 Protein ID WP_000125566.1
Coordinates 54796..55029 (+) Length 78 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECpv15279_RS00220 49870..50157 + 288 WP_000203741.1 ferredoxin-like protein FixX -
ECpv15279_RS00225 50215..51546 + 1332 WP_001183177.1 MFS transporter -
ECpv15279_RS00230 51654..52184 + 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
ECpv15279_RS00235 52177..54039 + 1863 WP_000377184.1 glutathione-regulated potassium-efflux system protein KefC -
ECpv15279_RS00240 54231..54710 + 480 WP_000624372.1 type 3 dihydrofolate reductase -
ECpv15279_RS00245 54796..55029 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
ECpv15279_RS00250 55032..55346 + 315 WP_001160975.1 CcdB family protein Toxin
ECpv15279_RS00255 55343..56191 - 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
ECpv15279_RS00260 56198..56575 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
ECpv15279_RS00265 56578..57399 - 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
ECpv15279_RS00270 57396..58385 - 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
ECpv15279_RS00275 58385..59671 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-103)

Antitoxin

(8-77)


Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T31794 WP_001160975.1 NZ_AP018488:55032-55346 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T31794 NZ_AP018488:55032-55346 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT31794 WP_000125566.1 NZ_AP018488:54796-55029 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT31794 NZ_AP018488:54796-55029 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References