Detailed information of TA system TA031781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1272950..1273867
• Replicon: chromosome
• Accession: NZ_AP018402
• Organism: Bacillus velezensis strain S141

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: BVS141_RS06695
• Protein ID: WP_032866111.1
• Coordinates: 1273121..1273867 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: -
• Locus tag: BVS141_RS06690
• Protein ID: WP_060561801.1
• Coordinates: 1272950..1273120 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVS141_RS06655	1268175..1269806	+	1632	WP_060561803.1	pyocin knob domain-containing protein	-
BVS141_RS06660	1269819..1270190	+	372	WP_060561802.1	hypothetical protein	-
BVS141_RS06665	1270195..1270392	+	198	WP_059367156.1	XkdX family protein	-
BVS141_RS06670	1270449..1271209	+	761	Protein_1232	phage portal protein	-
BVS141_RS06675	1271261..1271524	+	264	WP_003154815.1	hemolysin XhlA family protein	-
BVS141_RS06680	1271538..1271801	+	264	WP_003154813.1	phage holin	-
BVS141_RS06685	1271815..1272693	+	879	WP_043021264.1	N-acetylmuramoyl-L-alanine amidase	-
BVS141_RS06690	1272950..1273120	-	171	WP_060561801.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BVS141_RS06695	1273121..1273867	-	747	WP_032866111.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BVS141_RS06700	1273972..1274970	-	999	WP_007407255.1	inorganic phosphate transporter	-
BVS141_RS06705	1274983..1275600	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BVS141_RS06710	1275886..1277202	-	1317	WP_007610842.1	amino acid permease	-
BVS141_RS06715	1277525..1278475	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29095.60 Da
• Isoelectric Point: 4.7755

>T31781 WP_032866111.1 NZ_AP018402:c1273867-1273121 [Bacillus velezensis]
MLMFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T31781 NZ_AP018402:c1273867-1273121 [Bacillus velezensis]
ATGCTTATGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGATGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6690.77 Da
• Isoelectric Point: 10.9273

>AT31781 WP_060561801.1 NZ_AP018402:c1273120-1272950 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQLSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT31781 NZ_AP018402:c1273120-1272950 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGCTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA