Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1557150..1557818 Replicon chromosome
Accession NZ_AP018391
Organism Streptococcus pneumoniae strain MDRSPN001

Toxin (Protein)


Gene name hicA Uniprot ID C1C927
Locus tag MDRSPN_RS08080 Protein ID WP_001132282.1
Coordinates 1557639..1557818 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID G0IC64
Locus tag MDRSPN_RS08075 Protein ID WP_000961810.1
Coordinates 1557150..1557602 (-) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MDRSPN_RS08055 1553999..1555033 - 1035 WP_000747916.1 ATP-binding protein -
MDRSPN_RS08060 1555143..1555301 - 159 WP_000418159.1 7,8-dihydro-8-oxoguanine-triphosphatase -
MDRSPN_RS08065 1555282..1556370 - 1089 WP_000719711.1 M50 family metallopeptidase -
MDRSPN_RS08070 1556389..1556859 - 471 WP_000257114.1 DUF3013 family protein -
MDRSPN_RS08075 1557150..1557602 - 453 WP_000961810.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MDRSPN_RS08080 1557639..1557818 - 180 WP_001132282.1 type II toxin-antitoxin system HicA family toxin Toxin
MDRSPN_RS08085 1557955..1558134 - 180 WP_001048906.1 hypothetical protein -
MDRSPN_RS08095 1558299..1558538 - 240 WP_000818079.1 hypothetical protein -
MDRSPN_RS08105 1558808..1560079 + 1272 WP_001113206.1 replication-associated recombination protein A -
MDRSPN_RS08115 1560626..1561945 - 1320 WP_000502563.1 glycoside hydrolase family 32 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(17-114)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6713.95 Da        Isoelectric Point: 11.0174

>T31774 WP_001132282.1 NZ_AP018391:c1557818-1557639 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGELNKYTERGIRKQAGL

Download         Length: 180 bp

>T31774 NZ_AP018391:c1557818-1557639 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACTTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16659.46 Da        Isoelectric Point: 3.7220

>AT31774 WP_000961810.1 NZ_AP018391:c1557602-1557150 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Download         Length: 453 bp

>AT31774 NZ_AP018391:c1557602-1557150 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACTATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7SUI3

Antitoxin

Source ID Structure
PDB 5YRZ
AlphaFold DB G0IC64

References