Detailed information of TA system TA031768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta(toxin)
• Location: 8802..10453
• Replicon: plasmid pConjugative
• Accession: NZ_AP018378
• Organism: Neisseria gonorrhoeae strain FC428

Toxin (Protein)

• Gene name: ng_zeta1
• Uniprot ID: -
• Locus tag: EP178_RS13220
• Protein ID: WP_033911112.1
• Coordinates: 8802..10007 (+)
• Length: 402 a.a.

Antitoxin (Protein)

• Gene name: ng_epsilon1
• Uniprot ID: D5K9G8
• Locus tag: EP178_RS13225
• Protein ID: WP_003701903.1
• Coordinates: 10022..10453 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP178_RS13185	4390..4758	+	369	WP_003701920.1	hypothetical protein	-
EP178_RS13190	4742..5113	+	372	WP_003701919.1	hypothetical protein	-
EP178_RS13195	5117..6013	+	897	WP_003701917.1	hypothetical protein	-
EP178_RS13200	6165..6653	-	489	WP_014580055.1	hypothetical protein	-
EP178_RS13205	7123..7371	+	249	WP_014580056.1	hypothetical protein	-
EP178_RS13210	7368..8576	+	1209	WP_014580057.1	zeta toxin family protein	-
EP178_RS13215	8610..8795	+	186	WP_014580058.1	hypothetical protein	-
EP178_RS13220	8802..10007	+	1206	WP_033911112.1	zeta toxin family protein	Toxin
EP178_RS13225	10022..10453	+	432	WP_003701903.1	hypothetical protein	Antitoxin
EP178_RS13230	10505..11059	+	555	WP_003701901.1	recombinase family protein	-
EP178_RS13235	11169..11459	-	291	WP_003701899.1	virulence factor	-
EP178_RS13240	11562..12161	-	600	WP_003701898.1	TrbN protein	-
EP178_RS13245	12161..12739	-	579	WP_033911116.1	TrbM protein	-
EP178_RS13250	12726..14353	-	1628	Protein_17	P-type conjugative transfer protein TrbL	-
EP178_RS13255	14567..15343	-	777	WP_003701891.1	P-type conjugative transfer protein TrbJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39058	39058

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• KfrB domain (284-343)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 402 a.a.
• Molecular weight: 44747.59 Da
• Isoelectric Point: 7.2930

>T31768 WP_033911112.1 NZ_AP018378:8802-10007 [Neisseria gonorrhoeae]
MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQHADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHN
SAAVLSSTQFDEVLKRDEPQKKEVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
VSMRAERASDNTYKRFNEYGREASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQDRNSEFIDKFDDVIKMLSLGSQE
QILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESVFAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGI
YLLGQAKKAETGQTYSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLEYSAKKSIS
R

Nucleotide

• Length: 1206 bp

>T31768 NZ_AP018378:8802-10007 [Neisseria gonorrhoeae]
ATGGTAAAACTGTCTAGCGATATTAATCTGAGAGATTTTGGCAATAACGAATATTTGTCTTCCGTTCAGGATGAGGCCAT
CCGTTTTGCGACCGAACAAACAGACGAGATATTGTCTTTATATAGCCAACATGCAGATACAGAAGGCGGTAGATATGTTT
GTGCTGATACGTTTAAAGAGTTGTTTCCAGCTTTTGAGAACAAAGAAGACAGGGCAACGGTTAATAATGCCATCCACAAT
TCAGCAGCAGTTTTATCTTCTACCCAGTTTGACGAGGTACTGAAACGCGACGAGCCACAGAAGAAAGAAGTTATTTTCGT
TACGGGAATTCCGGGTTCGGGAAAAACTTCGACAGTAAAAAACATGATGATGCAGGATACCACCAAGCTGCTTTTTGAAG
GACAGCTTGCACGGCCGCAGTCTGCTTTTCGCAAGATTGAGCAGTGTCTGGAGCGTAATCTTGAAGTTACTATCGTAGCC
GTCAGCATGAGGGCTGAAAGGGCTTCCGACAATACTTACAAACGGTTTAACGAATACGGCAGGGAAGCCAGTATAGGTAT
TATGGCTGATATTCAGGCCAATCTTCCTGACGGACTCAAGCAAATCAGGGATAAGTTTGGTGATGCGGTAAAAATTGTCG
GTATCAACCAAGACAGGAACAGTGAATTTATCGACAAGTTCGATGATGTCATCAAAATGCTTTCCTTGGGGTCGCAAGAG
CAAATATTGGGCAGGTTGGCCGAGAAAATCCAGAGCGACTTCGACTCAGGAAAAATCAGCCGGGAATGCTTCAATCAGGC
CAAGGGCAGTATGGATCTTGAAAGTGTTTTCGCCAAAAAAGAGTATTCCCAGCAAAGGGTAGTTACTAACTCCAAGGGTG
TTACCCTAGAAACAAAGTCAGCAAACGAGTTGTGGTCTAAGGTGGAACAAATTCCTGTAACAGGCATGAAAGCCGGTATC
TATTTACTAGGTCAAGCTAAAAAAGCAGAGACTGGTCAAACTTATTCCGGAGAAATCATTTACAAGGATGCCGCTGCGGT
ATTTCAAAAAACCAAAAACGGGCTTGTCAGGCACAATGCAACACATAACGAAGAAAGACTGGCCAAATTGGTTGAGATAG
GGCAGAACGTTTCTATCGGAAGCAATAAGGGCAAATTGATTGTCAAGTCTTTGGAGTATTCCGCCAAAAAATCCATCAGC
AGATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15927.05 Da
• Isoelectric Point: 4.4211

>AT31768 WP_003701903.1 NZ_AP018378:10022-10453 [Neisseria gonorrhoeae]
MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLADILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVR
LSPFLNGCPHDLSLGAESLGKGESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ

Nucleotide

• Length: 432 bp

>AT31768 NZ_AP018378:10022-10453 [Neisseria gonorrhoeae]
ATGAAAGAAAAAATGCGGTTGATCACGGAAATTTCGCTGATTATCAGGGAACTGTCTTATTCCCGGCGCGGGTATCGGCC
TGAGAGTTATGCGGCTGCGCTGGATAATTTGACGATAATCGGAGATTTGGCCGACATTTTGCACAACATCGAGGCAGCGA
CAGGCAATCCATTTGTTCAAAGGATGGTTGATGAAAAACTGAGAAAGTTTGTTACCGATTTTCCGGCTTATAAGGTACGG
TTGTCTCCGTTTTTGAATGGCTGTCCCCATGACCTTTCGTTAGGAGCAGAATCGTTGGGTAAGGGGGAGAGTATCGCTGA
TGCCTTGGCCGTTCCTCCGGAAGAGGTAGATATTGCAGACATTGAGTTTGACCGTGTGCTTATCGAAGACGACCGTTGGA
CAGGAGCATATGATGGTGGTAACGCGCAATGA

Structures

Antitoxin

• AlphaFold DB: D5K9G8