Detailed information of TA system TA031759

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2224707..2225221
• Replicon: chromosome
• Accession: NZ_AP018349
• Organism: Staphylococcus aureus strain GN1

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: D8S83_RS11300
• Protein ID: WP_000581792.1
• Coordinates: 2224707..2224916 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: D8S83_RS11305
• Protein ID: WP_000138934.1
• Coordinates: 2224928..2225221 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8S83_RS11280	2220214..2221572	-	1359	WP_000611467.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
D8S83_RS11285	2221587..2222657	-	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
D8S83_RS11290	2222975..2224177	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
D8S83_RS11295	2224441..2224578	-	138	WP_000828354.1	hypothetical protein	-
D8S83_RS11300	2224707..2224916	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
D8S83_RS11305	2224928..2225221	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
D8S83_RS11310	2225387..2226871	+	1485	WP_020977447.1	cardiolipin synthase	-
D8S83_RS11315	2226899..2227546	+	648	WP_001187619.1	HD domain-containing protein	-
D8S83_RS11340	2228360..2229232	-	873	WP_000725802.1	membrane protein insertase YidC	-
D8S83_RS11345	2229318..2229959	-	642	WP_000483165.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T31759 WP_000581792.1 NZ_AP018349:c2224916-2224707 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T31759 NZ_AP018349:c2224916-2224707 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT31759 WP_000138934.1 NZ_AP018349:c2225221-2224928 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT31759 NZ_AP018349:c2225221-2224928 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: A0A0D1GBN9