Detailed information of TA system TA031758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2204963..2205492
• Replicon: chromosome
• Accession: NZ_AP018349
• Organism: Staphylococcus aureus strain GN1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D8S83_RS11210
• Protein ID: WP_000621175.1
• Coordinates: 2204963..2205325 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: D8S83_RS11215
• Protein ID: WP_000948331.1
• Coordinates: 2205322..2205492 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8S83_RS11185	2201941..2202711	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
D8S83_RS11190	2202686..2203165	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
D8S83_RS11195	2203167..2203493	-	327	WP_001052491.1	anti-sigma factor antagonist	-
D8S83_RS11200	2203612..2204613	-	1002	WP_120173206.1	PP2C family protein-serine/threonine phosphatase	-
D8S83_RS11210	2204963..2205325	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D8S83_RS11215	2205322..2205492	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
D8S83_RS11220	2205577..2206725	-	1149	WP_120173207.1	alanine racemase	-
D8S83_RS11225	2206791..2207150	-	360	WP_000581197.1	holo-ACP synthase	-
D8S83_RS11230	2207154..2207645	-	492	WP_001205916.1	PH domain-containing protein	-
D8S83_RS11235	2207632..2209215	-	1584	WP_001294642.1	PH domain-containing protein	-
D8S83_RS11240	2209208..2209687	-	480	WP_001287083.1	hypothetical protein	-
D8S83_RS11245	2209896..2210456	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T31758 WP_000621175.1 NZ_AP018349:c2205325-2204963 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T31758 NZ_AP018349:c2205325-2204963 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT31758 WP_000948331.1 NZ_AP018349:c2205492-2205322 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT31758 NZ_AP018349:c2205492-2205322 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1