Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tsbAT/-
Location 2024728..2025504 Replicon chromosome
Accession NZ_AP018349
Organism Staphylococcus aureus strain GN1

Toxin (Protein)


Gene name tsbT Uniprot ID X5E2E6
Locus tag D8S83_RS10135 Protein ID WP_000031108.1
Coordinates 2024728..2024880 (-) Length 51 a.a.

Antitoxin (Protein)


Gene name tsbA Uniprot ID A0A0D1IYN8
Locus tag D8S83_RS10140 Protein ID WP_001251219.1
Coordinates 2024905..2025504 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8S83_RS10115 2020808..2021629 + 822 WP_020977385.1 RluA family pseudouridine synthase -
D8S83_RS10120 2022093..2023478 - 1386 WP_020977386.1 class II fumarate hydratase -
D8S83_RS10125 2023674..2024069 - 396 WP_000901023.1 hypothetical protein -
D8S83_RS10135 2024728..2024880 - 153 WP_000031108.1 hypothetical protein Toxin
D8S83_RS10140 2024905..2025504 - 600 WP_001251219.1 glucosamine-6-phosphate isomerase Antitoxin
D8S83_RS10145 2025664..2026134 - 471 WP_000181394.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
D8S83_RS10150 2026139..2027266 - 1128 WP_020977387.1 tRNA epoxyqueuosine(34) reductase QueG -
D8S83_RS10155 2027417..2028139 - 723 WP_000590809.1 amino acid ABC transporter ATP-binding protein -
D8S83_RS10160 2028132..2029589 - 1458 WP_020977388.1 ABC transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Glucosamine-6-phosphate isomerase

(124-196)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5936.28 Da        Isoelectric Point: 3.8962

>T31756 WP_000031108.1 NZ_AP018349:c2024880-2024728 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Download         Length: 153 bp

>T31756 NZ_AP018349:c2024880-2024728 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22357.50 Da        Isoelectric Point: 5.1445

>AT31756 WP_001251219.1 NZ_AP018349:c2025504-2024905 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNIEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Download         Length: 600 bp

>AT31756 NZ_AP018349:c2025504-2024905 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTTGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X5E2E6

Antitoxin

Source ID Structure
AlphaFold DB A0A0D1IYN8

References