Detailed information of TA system TA031743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2069101..2069789
• Replicon: chromosome
• Accession: NZ_AP018335
• Organism: Ureibacillus thermosphaericus strain A1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: UtA1_RS09930
• Protein ID: WP_096550891.1
• Coordinates: 2069101..2069487 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: UtA1_RS09935
• Protein ID: WP_096550892.1
• Coordinates: 2069487..2069789 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UtA1_RS09920	2065788..2067059	-	1272	WP_096550285.1	IS4 family transposase	-
UtA1_RS09925	2067452..2068723	-	1272	WP_096550285.1	IS4 family transposase	-
UtA1_RS09930	2069101..2069487	-	387	WP_096550891.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
UtA1_RS09935	2069487..2069789	-	303	WP_096550892.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
UtA1_RS09945	2070666..2071058	+	393	WP_096552478.1	hypothetical protein	-
UtA1_RS09955	2071429..2072421	+	993	WP_096550893.1	WYL domain-containing protein	-
UtA1_RS09960	2073343..2074698	+	1356	WP_096550894.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-115)

Antitoxin

• Antitoxin PemI-like (21-98)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14389.51 Da
• Isoelectric Point: 5.1886

>T31743 WP_096550891.1 NZ_AP018335:c2069487-2069101 [Ureibacillus thermosphaericus]
MSKETLNRGDFIILNFHPQAGHEQAGTRIAIVLSPRAFNEATNFTAVCPITNQLKGYPYEVALPKEGIFVKDGNPITGSI
LADQVKTLDLSARYYKILKKYNPQDEQIEIINEIIEECLAKIETYLGF

Nucleotide

• Length: 387 bp

>T31743 NZ_AP018335:c2069487-2069101 [Ureibacillus thermosphaericus]
ATGTCGAAAGAAACGTTAAATCGAGGAGATTTTATTATTTTAAACTTTCATCCGCAAGCCGGCCATGAACAAGCCGGTAC
AAGGATTGCTATTGTTTTGTCACCAAGAGCTTTTAATGAAGCAACGAATTTTACGGCTGTTTGTCCAATCACAAATCAAC
TAAAAGGTTATCCGTATGAAGTTGCATTGCCAAAAGAAGGCATCTTCGTTAAAGATGGCAATCCAATTACCGGATCGATT
TTGGCTGACCAAGTTAAAACATTAGATTTATCTGCACGTTACTATAAAATTTTAAAGAAGTATAATCCCCAAGATGAACA
AATTGAAATCATTAATGAAATCATTGAAGAATGTTTAGCCAAAATAGAAACATATTTAGGGTTCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11278.96 Da
• Isoelectric Point: 9.5402

>AT31743 WP_096550892.1 NZ_AP018335:c2069789-2069487 [Ureibacillus thermosphaericus]
MSADKYLGNQKNLGGNHMATVTAQKWGNSLGIRIPKEAADKLGIKQGSEMILRINESDGIITLQPKKSRKKYNLEELVFQ
ITPEKRHEEIDFGIEGRELI

Nucleotide

• Length: 303 bp

>AT31743 NZ_AP018335:c2069789-2069487 [Ureibacillus thermosphaericus]
ATGAGTGCAGATAAGTATTTAGGCAATCAAAAAAATTTAGGAGGAAATCACATGGCAACCGTTACTGCACAAAAATGGGG
AAACAGTTTGGGCATTCGGATCCCAAAAGAGGCAGCTGATAAATTAGGTATCAAACAGGGGTCGGAAATGATATTACGAA
TAAATGAAAGTGATGGAATCATCACATTGCAACCAAAAAAAAGTCGTAAAAAATACAATTTAGAAGAATTGGTATTCCAA
ATCACTCCGGAGAAAAGACATGAAGAAATTGACTTTGGAATTGAAGGACGTGAATTGATTTAA