Detailed information of TA system TA031734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicA-vapB/HicA-DUF433
• Location: 8322405..8322735
• Replicon: chromosome
• Accession: NZ_AP018255
• Organism: Calothrix sp. NIES-4071

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CDC41_RS36640
• Protein ID: WP_096691306.1
• Coordinates: 8322613..8322735 (+)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CDC41_RS36635
• Protein ID: WP_096694418.1
• Coordinates: 8322405..8322626 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC41_RS36615	8317553..8318452	-	900	WP_096691303.1	heterocyst differentiation master regulator HetR	-
CDC41_RS36620	8319041..8320657	-	1617	WP_096694417.1	EamA family transporter	-
CDC41_RS36625	8321532..8321801	+	270	WP_096691304.1	hypothetical protein	-
CDC41_RS36630	8321629..8322387	+	759	WP_096691305.1	GUN4 domain-containing protein	-
CDC41_RS36635	8322405..8322626	+	222	WP_096694418.1	DUF433 domain-containing protein	Antitoxin
CDC41_RS36640	8322613..8322735	+	123	WP_096691306.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDC41_RS36645	8322865..8323059	+	195	WP_096691307.1	type II toxin-antitoxin system HicB family antitoxin	-
CDC41_RS36650	8323191..8324444	-	1254	WP_096694419.1	inorganic phosphate transporter	-
CDC41_RS36655	8324556..8324780	+	225	Protein_7264	hypothetical protein	-
CDC41_RS36660	8325208..8326590	+	1383	WP_096691308.1	alkaline phosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-39)

Antitoxin

• Protein of unknown function DUF433 (7-62)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4723.44 Da
• Isoelectric Point: 10.7295

>T31734 WP_096691306.1 NZ_AP018255:8322613-8322735 [Calothrix sp. NIES-4071]
MKVREVIKRLEADGWYLDRTRGSHRQYKHPDKPGTVTVKA

Nucleotide

• Length: 123 bp

>T31734 NZ_AP018255:8322613-8322735 [Calothrix sp. NIES-4071]
ATGAAAGTACGTGAAGTAATCAAGCGATTAGAAGCAGATGGTTGGTATCTTGATAGAACTAGGGGTAGCCATCGACAGTA
TAAACATCCTGATAAACCTGGTACTGTAACAGTAAAGGCGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8303.53 Da
• Isoelectric Point: 5.0019

>AT31734 WP_096694418.1 NZ_AP018255:8322405-8322626 [Calothrix sp. NIES-4071]
MSFRDIITIEPDKRGGKPCIRRMRITVYDVLSWLAAGMSHAQIMDDFPELTEEDIRACLKFAAVQVNNSNEST

Nucleotide

• Length: 222 bp

>AT31734 NZ_AP018255:8322405-8322626 [Calothrix sp. NIES-4071]
ATGAGCTTCCGCGACATTATAACAATTGAACCTGATAAGCGAGGCGGTAAGCCCTGTATTCGACGCATGAGAATCACAGT
GTATGATGTTTTAAGCTGGTTAGCAGCAGGGATGTCTCATGCTCAAATAATGGACGATTTTCCTGAATTAACAGAAGAGG
ATATTCGAGCTTGCTTAAAATTTGCTGCTGTACAAGTTAATAACAGTAATGAAAGTACGTGA