Detailed information of TA system TA031733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 8030185..8030737
• Replicon: chromosome
• Accession: NZ_AP018255
• Organism: Calothrix sp. NIES-4071

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CDC41_RS35325
• Protein ID: WP_096691084.1
• Coordinates: 8030185..8030526 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CDC41_RS35330
• Protein ID: WP_096691085.1
• Coordinates: 8030513..8030737 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC41_RS35285	8026485..8026613	-	129	Protein_6990	PIN domain nuclease	-
CDC41_RS35290	8026898..8027225	+	328	Protein_6991	XisI protein	-
CDC41_RS35295	8027305..8027934	-	630	Protein_6992	hypothetical protein	-
CDC41_RS35300	8028108..8028347	+	240	WP_096691081.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CDC41_RS35305	8028350..8028755	+	406	Protein_6994	type II toxin-antitoxin system death-on-curing family toxin	-
CDC41_RS35310	8028761..8028979	+	219	WP_096694398.1	hypothetical protein	-
CDC41_RS35315	8029265..8029672	-	408	WP_096691082.1	type II toxin-antitoxin system VapC family toxin	-
CDC41_RS35320	8029675..8029890	-	216	WP_096691083.1	hypothetical protein	-
CDC41_RS35325	8030185..8030526	-	342	WP_096691084.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CDC41_RS35330	8030513..8030737	-	225	WP_096691085.1	ribbon-helix-helix domain-containing protein	Antitoxin
CDC41_RS35335	8030957..8031376	+	420	WP_096691086.1	fatty-acid synthase	-
CDC41_RS35340	8031361..8031699	+	339	WP_096691087.1	XisI protein	-
CDC41_RS35345	8032176..8032519	-	344	Protein_7002	MazF family transcriptional regulator	-
CDC41_RS35350	8032506..8032730	-	225	WP_096691088.1	ribbon-helix-helix domain-containing protein	-
CDC41_RS35355	8033192..8034391	+	1200	WP_096694399.1	restriction endonuclease subunit R	-
CDC41_RS35360	8034603..8034923	-	321	WP_096691089.1	XRE family transcriptional regulator	-
CDC41_RS35365	8034920..8035279	-	360	WP_096691090.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12457.45 Da
• Isoelectric Point: 9.7314

>T31733 WP_096691084.1 NZ_AP018255:c8030526-8030185 [Calothrix sp. NIES-4071]
MKRGDIFYADLSPVVGSEMGKRRPVLIVSNNANNRTSTTVTILPLTSNVTRVYPFEVLIMPEDSNLSKPSKVQAQQVRTI
SRERITSDFVGSLSEELMVLIDAALKLHLGLGL

Nucleotide

• Length: 342 bp

>T31733 NZ_AP018255:c8030526-8030185 [Calothrix sp. NIES-4071]
ATGAAACGTGGTGATATTTTTTACGCCGACCTTAGTCCTGTGGTGGGTTCAGAAATGGGTAAACGTCGTCCGGTCTTGAT
AGTAAGCAATAATGCAAATAATCGTACTTCCACCACAGTAACGATATTGCCGCTTACTTCCAACGTAACGCGCGTTTATC
CATTTGAAGTTTTGATTATGCCAGAGGATAGCAATTTATCTAAACCATCTAAAGTACAAGCACAACAAGTGCGAACAATT
TCTAGAGAACGTATAACTAGCGATTTTGTGGGTAGTTTAAGCGAAGAGTTGATGGTTTTAATTGATGCTGCGTTAAAACT
ACATTTGGGACTAGGTTTATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8392.50 Da
• Isoelectric Point: 4.2472

>AT31733 WP_096691085.1 NZ_AP018255:c8030737-8030513 [Calothrix sp. NIES-4071]
MNVEKLSISLPPSLVEFIENYKLSKGCKSRSQVIEEALELLRNRELEERLRLASAEVDSAWDVTVADGLADETW

Nucleotide

• Length: 225 bp

>AT31733 NZ_AP018255:c8030737-8030513 [Calothrix sp. NIES-4071]
ATGAACGTAGAGAAATTATCTATATCCTTGCCACCATCTTTAGTAGAGTTTATTGAAAACTATAAACTGAGCAAAGGATG
TAAGTCCCGTTCTCAAGTAATTGAGGAAGCATTGGAGTTACTGCGAAACCGGGAACTAGAAGAGCGATTAAGACTAGCTT
CTGCTGAGGTTGACAGTGCTTGGGATGTGACAGTCGCGGATGGGTTAGCAGATGAAACGTGGTGA