Detailed information of TA system TA031729

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 3764287..3765192
• Replicon: chromosome
• Accession: NZ_AP018255
• Organism: Calothrix sp. NIES-4071

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: CDC41_RS16000
• Protein ID: WP_096687804.1
• Coordinates: 3764749..3765192 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: CDC41_RS15995
• Protein ID: WP_096687803.1
• Coordinates: 3764287..3764739 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC41_RS15990	3760085..3760357	+	273	WP_096694147.1	CRISPR-associated endonuclease Cas2	-
CDC41_RS15995	3764287..3764739	+	453	WP_096687803.1	nucleotidyltransferase domain-containing protein	Antitoxin
CDC41_RS16000	3764749..3765192	+	444	WP_096687804.1	DUF86 domain-containing protein	Toxin
CDC41_RS16005	3765399..3768524	-	3126	WP_096687805.1	CHAT domain-containing protein	-
CDC41_RS16010	3768940..3769317	+	378	WP_096687806.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (13-131)

Antitoxin

• Polymerase beta, nucleotidyltransferase (23-113)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16868.37 Da
• Isoelectric Point: 4.6251

>T31729 WP_096687804.1 NZ_AP018255:3764749-3765192 [Calothrix sp. NIES-4071]
MKREPVTSRLVLIIKYLNRLREFESVSVDEYLNSFDYQLIAERLIQLLVDVASDINTYILVQLHNIVPSAYYDSFVEAGK
KGIVTRELALGLAASAGMRNRLVHQYEVIDNRIVFSAIPIALDQYQLYVQEITSFLDSLEITEHDSI

Nucleotide

• Length: 444 bp

>T31729 NZ_AP018255:3764749-3765192 [Calothrix sp. NIES-4071]
ATGAAACGAGAACCTGTTACAAGTAGACTTGTGTTAATAATCAAGTATCTAAATAGGTTGCGTGAGTTTGAATCGGTGAG
TGTTGATGAGTATTTAAATAGTTTTGACTATCAACTGATTGCTGAAAGGTTAATCCAGTTATTAGTTGATGTTGCTTCAG
ATATTAATACTTATATATTGGTGCAATTACATAATATTGTTCCATCAGCTTACTATGATTCTTTTGTTGAGGCTGGAAAA
AAGGGTATTGTTACAAGAGAACTAGCTTTAGGGCTTGCGGCTTCAGCAGGAATGCGAAATCGCTTGGTTCATCAGTATGA
GGTGATTGACAATCGTATCGTTTTTTCGGCTATCCCTATAGCTTTAGATCAATATCAGTTATATGTACAGGAAATTACAA
GTTTCTTAGATTCTTTGGAAATAACAGAGCATGACTCAATCTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 17472.22 Da
• Isoelectric Point: 7.2148

>AT31729 WP_096687803.1 NZ_AP018255:3764287-3764739 [Calothrix sp. NIES-4071]
MVNKTPTVAELRELSLRLPENIPYVKMLVLFGSRARGDIHANSDWDFAVICDENQRLDYVKDNPFLWFELPAKVGEILQI
NSDIIDVVELDHCSPLMGYHVARDAKVLYESKPESFLRFQCKAWKIYADTAKFRKAERESIKLWLNKRGL

Nucleotide

• Length: 453 bp

>AT31729 NZ_AP018255:3764287-3764739 [Calothrix sp. NIES-4071]
ATGGTAAATAAAACACCAACAGTTGCAGAATTGAGAGAACTTTCTTTGCGACTTCCAGAGAACATTCCGTATGTTAAAAT
GCTAGTTTTATTCGGTTCGAGAGCTAGAGGTGATATTCATGCTAATAGTGATTGGGATTTTGCTGTAATTTGTGATGAGA
ACCAACGTTTAGATTATGTAAAGGATAATCCTTTTTTGTGGTTTGAGCTACCTGCAAAAGTTGGCGAGATTCTCCAAATC
AATAGCGATATTATAGATGTGGTAGAACTTGATCATTGTTCGCCATTGATGGGTTATCATGTAGCCCGTGATGCTAAGGT
TTTATATGAAAGTAAACCAGAGAGTTTTCTTAGATTTCAATGCAAAGCCTGGAAGATTTATGCTGATACAGCAAAATTCC
GCAAAGCTGAAAGAGAATCTATAAAACTCTGGTTGAATAAAAGAGGGTTATAA