Detailed information of TA system TA031727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3017318..3018361
• Replicon: chromosome
• Accession: NZ_AP018255
• Organism: Calothrix sp. NIES-4071

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CDC41_RS12655
• Protein ID: WP_096687026.1
• Coordinates: 3017861..3018361 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: CDC41_RS12650
• Protein ID: WP_096687024.1
• Coordinates: 3017318..3017788 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC41_RS12630	3013126..3013797	-	672	WP_096687016.1	hypothetical protein	-
CDC41_RS12635	3013794..3014696	-	903	WP_096687018.1	hypothetical protein	-
CDC41_RS12640	3015126..3016274	+	1149	WP_096687020.1	alpha/beta hydrolase	-
CDC41_RS12645	3016309..3017154	+	846	WP_096687022.1	SDR family NAD(P)-dependent oxidoreductase	-
CDC41_RS12650	3017318..3017788	+	471	WP_096687024.1	helix-turn-helix domain-containing protein	Antitoxin
CDC41_RS12655	3017861..3018361	+	501	WP_096687026.1	PIN domain-containing protein	Toxin
CDC41_RS12660	3018452..3019438	-	987	WP_096687028.1	phosphotransferase	-
CDC41_RS12665	3019603..3019917	+	315	WP_096687030.1	hypothetical protein	-
CDC41_RS12670	3020033..3020725	-	693	WP_096687032.1	helix-turn-helix transcriptional regulator	-
CDC41_RS12675	3020835..3021266	+	432	WP_096694090.1	hypothetical protein	-
CDC41_RS12680	3021329..3022162	+	834	WP_096687034.1	sulfurtransferase	-
CDC41_RS12685	3022268..3023116	+	849	WP_096687036.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-90)

Antitoxin

• Helix-turn-helix domain, group 17 (86-133)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18735.60 Da
• Isoelectric Point: 6.2345

>T31727 WP_096687026.1 NZ_AP018255:3017861-3018361 [Calothrix sp. NIES-4071]
MWLALTNLFKARWTEEIHLEWTRNVLKNRPDLTLEQLTRTKNLMNANVRDCLVTGYESLITAIELPDPDDRHVLAAAIRT
GADVIVTFNLSDFPFLALEPYGVKAVHPDNFIQNLIDLNPEAVCAAAQKQRQTLKNPSKTIDEYLDTLLKSGLKQTVAML
KSLYAG

Nucleotide

• Length: 501 bp

>T31727 NZ_AP018255:3017861-3018361 [Calothrix sp. NIES-4071]
ATGTGGCTAGCACTCACCAACTTATTCAAAGCTCGGTGGACAGAGGAAATTCATTTAGAATGGACTAGAAATGTTTTGAA
AAACCGTCCTGACTTAACACTTGAACAGTTAACTAGGACAAAAAATCTTATGAATGCTAATGTCAGGGATTGTTTAGTGA
CTGGTTACGAATCTTTGATTACAGCTATTGAACTTCCCGATCCTGATGATCGTCATGTTTTAGCAGCAGCTATTCGCACC
GGAGCCGACGTTATAGTTACTTTTAATTTAAGTGACTTTCCTTTCTTAGCTTTAGAGCCTTATGGCGTTAAAGCAGTTCA
CCCAGATAATTTTATCCAGAATTTGATTGACCTGAACCCAGAAGCCGTTTGTGCAGCTGCCCAAAAACAACGCCAGACTC
TTAAAAATCCATCTAAAACAATAGATGAGTATCTTGATACGCTTCTTAAATCAGGGCTTAAGCAAACAGTAGCAATGTTA
AAATCGTTGTATGCAGGCTAG

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17161.67 Da
• Isoelectric Point: 5.2605

>AT31727 WP_096687024.1 NZ_AP018255:3017318-3017788 [Calothrix sp. NIES-4071]
MTLSINQKVQQLALTNEDANIAKASSQILESYLQKNLSYGVFKLISNDTEGETVTIPAAALNLLTEILTQMAAGNAVSVV
SIKKELTTQEAADILQVSRPYLVELLESGKIPYRKVGSRRRVLTQDVISYKNRIDAARMETLAELSAQAQELNMGY

Nucleotide

• Length: 471 bp

>AT31727 NZ_AP018255:3017318-3017788 [Calothrix sp. NIES-4071]
ATGACACTCTCTATAAATCAAAAGGTTCAACAGTTAGCACTTACAAACGAAGATGCGAATATAGCTAAAGCTAGCAGCCA
AATTTTGGAATCCTACTTACAAAAAAACCTTTCTTATGGGGTATTCAAGCTGATATCAAATGACACAGAAGGTGAAACTG
TAACCATACCAGCAGCGGCATTAAACTTACTTACAGAAATCTTAACACAAATGGCTGCTGGTAACGCCGTTAGCGTAGTT
TCGATAAAAAAGGAACTTACTACCCAAGAGGCAGCTGATATATTACAAGTTTCCCGTCCTTATTTAGTAGAATTACTAGA
ATCTGGAAAAATTCCCTATCGTAAAGTCGGTTCGCGTCGCCGTGTTTTGACTCAAGATGTAATCAGTTATAAAAATCGTA
TAGATGCAGCACGAATGGAAACCTTAGCTGAGTTATCAGCACAAGCCCAAGAACTTAACATGGGGTACTAG