Detailed information of TA system TA031726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1474703..1475298
• Replicon: chromosome
• Accession: NZ_AP018255
• Organism: Calothrix sp. NIES-4071

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CDC41_RS06210
• Protein ID: WP_096684947.1
• Coordinates: 1474942..1475298 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CDC41_RS06205
• Protein ID: WP_096684946.1
• Coordinates: 1474703..1474942 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC41_RS06195	1471075..1472979	+	1905	WP_096684944.1	GTPase family protein	-
CDC41_RS06200	1473620..1474461	+	842	Protein_1236	hypothetical protein	-
CDC41_RS06205	1474703..1474942	+	240	WP_096684946.1	CopG family transcriptional regulator	Antitoxin
CDC41_RS06210	1474942..1475298	+	357	WP_096684947.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CDC41_RS06215	1475448..1475825	-	378	WP_096684949.1	cupin domain-containing protein	-
CDC41_RS06220	1476491..1477534	+	1044	WP_096684950.1	low specificity L-threonine aldolase	-
CDC41_RS06225	1477742..1478305	-	564	WP_096693889.1	helix-turn-helix transcriptional regulator	-
CDC41_RS06230	1479252..1479812	-	561	WP_096693890.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13301.32 Da
• Isoelectric Point: 6.9485

>T31726 WP_096684947.1 NZ_AP018255:1474942-1475298 [Calothrix sp. NIES-4071]
MPKGNLTYKRGEIRFCKLDPTVGTELRKTRSCLIIQNDIMNQYGSLTIVMPFRPGSKNAPYIVNVVASPVNGLDQDRYID
VGQIRSIDNSRVLGLVGVLEEEYWESIHTALNIVLEFS

Nucleotide

• Length: 357 bp

>T31726 NZ_AP018255:1474942-1475298 [Calothrix sp. NIES-4071]
ATGCCTAAAGGGAACTTAACTTATAAACGCGGAGAAATACGCTTCTGTAAACTTGACCCAACAGTAGGGACAGAGTTGCG
AAAAACACGCTCTTGCCTAATTATTCAGAACGATATAATGAATCAGTACGGTTCGTTAACAATTGTAATGCCATTTAGAC
CAGGAAGTAAAAATGCTCCTTATATAGTTAATGTCGTAGCATCTCCTGTAAATGGTTTAGACCAAGACCGTTACATTGAT
GTTGGACAAATTCGCAGTATTGATAATAGCCGAGTTCTTGGCTTAGTAGGAGTATTGGAAGAAGAATATTGGGAATCAAT
TCATACAGCATTAAATATAGTACTTGAATTTTCCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8767.77 Da
• Isoelectric Point: 4.0831

>AT31726 WP_096684946.1 NZ_AP018255:1474703-1474942 [Calothrix sp. NIES-4071]
MAKKVTITLDEDILAFVDKRAASNGETANRSGYINDVLSQHRRKVLEAEMIAALTEDANDPEYQEEIALWDCVVGDGIE

Nucleotide

• Length: 240 bp

>AT31726 NZ_AP018255:1474703-1474942 [Calothrix sp. NIES-4071]
TTGGCTAAAAAAGTAACAATCACACTTGACGAAGACATTTTGGCTTTTGTGGATAAGCGAGCTGCCTCTAATGGAGAAAC
TGCTAACCGTAGTGGGTATATCAACGATGTTTTAAGTCAACACCGTCGTAAAGTCTTAGAAGCAGAAATGATTGCAGCAT
TAACTGAAGATGCTAATGACCCAGAGTATCAGGAGGAAATAGCACTTTGGGATTGTGTTGTTGGGGATGGAATTGAATAA