Detailed information of TA system TA031723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-UPF0150
• Location: 3436074..3436504
• Replicon: chromosome
• Accession: NZ_AP018254
• Organism: Calothrix sp. NIES-3974

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CDC37_RS13815
• Protein ID: WP_096622537.1
• Coordinates: 3436074..3436298 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G6FUJ8
• Locus tag: CDC37_RS13820
• Protein ID: WP_009457224.1
• Coordinates: 3436298..3436504 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDC37_RS13800	3431789..3432964	+	1176	WP_096622536.1	IscS subfamily cysteine desulfurase	-
CDC37_RS13805	3434000..3434806	+	807	WP_096626213.1	Nif3-like dinuclear metal center hexameric protein	-
CDC37_RS13810	3435006..3435285	-	280	Protein_2736	hypothetical protein	-
CDC37_RS13815	3436074..3436298	-	225	WP_096622537.1	type II toxin-antitoxin system HicA family toxin	Toxin
CDC37_RS13820	3436298..3436504	-	207	WP_009457224.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CDC37_RS13825	3436595..3436978	-	384	WP_096622538.1	type II toxin-antitoxin system death-on-curing family toxin	-
CDC37_RS13830	3436978..3437175	-	198	WP_096622539.1	DNA-binding protein	-
CDC37_RS13835	3437725..3438255	-	531	WP_096622540.1	GNAT family N-acetyltransferase	-
CDC37_RS24350	3438281..3438418	-	138	WP_157780748.1	hypothetical protein	-
CDC37_RS13840	3438498..3438827	-	330	WP_096622541.1	type II toxin-antitoxin system PemK/MazF family toxin	-
CDC37_RS13845	3438805..3439008	-	204	WP_096622542.1	antitoxin family protein	-
CDC37_RS13855	3439526..3439744	+	219	WP_096622543.1	DUF433 domain-containing protein	-
CDC37_RS13860	3439746..3440069	+	324	Protein_2746	DUF5615 family PIN-like protein	-
CDC37_RS13865	3440092..3440505	-	414	WP_096622544.1	GIY-YIG nuclease family protein	-
CDC37_RS13870	3440667..3441311	-	645	WP_096622545.1	NADPH-dependent F420 reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-65)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-58)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8108.39 Da
• Isoelectric Point: 6.4628

>T31723 WP_096622537.1 NZ_AP018254:c3436298-3436074 [Calothrix sp. NIES-3974]
MGSALPVMSGREVVRVFESLGWEAVRQTGSHIIMTKDEELVTLSIPDHREVAKGTLRSLIRTAGLTVEEFVAAK

Nucleotide

• Length: 225 bp

>T31723 NZ_AP018254:c3436298-3436074 [Calothrix sp. NIES-3974]
ATGGGTTCTGCTTTACCTGTCATGAGTGGGCGCGAAGTCGTGCGCGTCTTTGAGTCGTTAGGTTGGGAGGCTGTACGGCA
GACGGGTAGCCACATCATTATGACGAAGGACGAGGAGCTTGTCACACTTTCAATTCCTGATCACCGTGAAGTAGCAAAAG
GAACTTTAAGAAGTTTGATTCGCACTGCTGGATTAACCGTAGAGGAATTTGTTGCTGCTAAATAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7495.56 Da
• Isoelectric Point: 4.1435

>AT31723 WP_009457224.1 NZ_AP018254:c3436504-3436298 [Calothrix sp. NIES-3974]
MRFSVTLDRDEDGIWVVECPSIPGCVSQGQTKQEALENIKDAIAACLQVRAERGMPLTVETYQVEVVA

Nucleotide

• Length: 207 bp

>AT31723 NZ_AP018254:c3436504-3436298 [Calothrix sp. NIES-3974]
ATGAGATTTAGTGTAACGCTCGATCGCGATGAGGATGGAATTTGGGTTGTAGAGTGTCCTAGCATTCCGGGATGTGTGAG
TCAAGGTCAGACGAAACAGGAAGCCCTTGAAAATATCAAAGATGCGATCGCAGCGTGTCTACAGGTTCGGGCAGAGCGTG
GAATGCCGCTGACAGTAGAAACCTATCAGGTTGAGGTCGTTGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Z4NKE1