Detailed information of TA system TA031721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3586287..3587323
• Replicon: chromosome
• Accession: NZ_AP018203
• Organism: Leptolyngbya boryana NIES-2135

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CA740_RS17135
• Protein ID: WP_017288215.1
• Coordinates: 3586287..3586853 (-)
• Length: 189 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A1Z4JIQ6
• Locus tag: CA740_RS17140
• Protein ID: WP_017288216.1
• Coordinates: 3586859..3587323 (-)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA740_RS17110	3581308..3581637	+	330	WP_017288210.1	hypothetical protein	-
CA740_RS17115	3583041..3584138	-	1098	WP_017288211.1	PAS domain-containing protein	-
CA740_RS17120	3584409..3584606	-	198	WP_081614740.1	hypothetical protein	-
CA740_RS17125	3584632..3584982	-	351	WP_017288212.1	hypothetical protein	-
CA740_RS17130	3585282..3585914	-	633	WP_017288213.1	hypothetical protein	-
CA740_RS17135	3586287..3586853	-	567	WP_017288215.1	PIN domain-containing protein	Toxin
CA740_RS17140	3586859..3587323	-	465	WP_017288216.1	helix-turn-helix domain-containing protein	Antitoxin
CA740_RS17145	3587707..3587910	-	204	WP_081614741.1	hypothetical protein	-
CA740_RS17155	3588387..3591095	-	2709	WP_017288219.1	type I DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• SinI-like, DNA-binding domain (83-128)

Sequences

Toxin

Protein

• Length: 189 a.a.
• Molecular weight: 21641.89 Da
• Isoelectric Point: 5.6857

>T31721 WP_017288215.1 NZ_AP018203:c3586853-3586287 [Leptolyngbya boryana NIES-2135]
MSNFTAVYDACVLYPAPLRDFLMELALTDLFRARWSESIHQEWIRNVLKSRPDLSAEQLNKTKELMNSHVRDSVVTNYET
LIPAIELPDPDDRHVLAAAIRCNASVIVTFNLKDFPADTLKLYDIDAQHPDEFILYLLDLKPLAVCTAAERQRQRLKKPP
KDPEEYLGTLLRQGLPQTVSRLRESCYL

Nucleotide

• Length: 567 bp

>T31721 NZ_AP018203:c3586853-3586287 [Leptolyngbya boryana NIES-2135]
ATGTCTAATTTCACGGCTGTTTACGATGCGTGTGTTCTCTATCCTGCTCCACTACGCGACTTTTTGATGGAACTTGCTCT
AACTGACTTATTTAGAGCAAGGTGGTCAGAATCTATTCACCAAGAATGGATTCGGAATGTTCTTAAGAGCCGTCCTGATC
TTTCGGCTGAGCAACTTAATAAGACTAAGGAATTAATGAACAGTCATGTTCGAGATTCAGTAGTGACGAATTATGAAACA
TTAATTCCAGCGATTGAGCTTCCCGACCCGGATGATCGTCATGTGCTTGCTGCTGCTATTCGCTGTAACGCCAGTGTCAT
TGTCACGTTTAATTTGAAAGACTTTCCCGCAGACACGTTAAAGCTATATGACATTGATGCACAGCATCCAGATGAGTTCA
TTCTATATCTTCTAGATTTGAAGCCTCTGGCGGTTTGCACAGCAGCAGAGAGGCAGCGCCAACGCCTTAAAAAACCTCCA
AAAGATCCAGAAGAATATTTAGGAACTTTGCTTCGACAGGGACTACCTCAAACAGTTTCTCGCTTGCGAGAATCGTGTTA
TCTCTGA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 17200.86 Da
• Isoelectric Point: 6.2467

>AT31721 WP_017288216.1 NZ_AP018203:c3587323-3586859 [Leptolyngbya boryana NIES-2135]
MTITLQKSSPPTLEDIRLAKESSSRLARSIPSHESTSKISVIQGEATGETIVLPTAALQMLVEILAQMAQGNIVTLFPIH
AELTTQEAADLLKVSRPYLVKLLEDKALPFRKVGKHRRILFQDLMEYKNREDSLRSNALDELVAQAQELNMGYE

Nucleotide

• Length: 465 bp

>AT31721 NZ_AP018203:c3587323-3586859 [Leptolyngbya boryana NIES-2135]
ATGACAATTACATTGCAGAAGTCCAGTCCTCCGACCCTAGAAGATATCCGTTTGGCGAAAGAAAGCAGCAGTAGGCTTGC
TCGATCCATTCCAAGTCATGAATCGACGAGTAAGATCAGCGTGATCCAGGGCGAGGCAACTGGTGAGACGATCGTATTGC
CGACAGCAGCGCTTCAGATGTTGGTTGAAATCCTTGCGCAGATGGCGCAGGGGAATATTGTTACTCTTTTTCCAATCCAC
GCAGAGCTTACAACTCAGGAAGCGGCTGATTTACTCAAAGTGTCCCGACCTTATCTAGTTAAGCTTCTTGAGGACAAGGC
ATTGCCTTTTAGAAAAGTTGGAAAGCATCGGCGCATTCTTTTCCAAGATCTTATGGAATACAAAAACCGTGAAGACAGCC
TTAGAAGTAATGCGCTGGATGAGCTTGTAGCTCAAGCCCAAGAGCTAAACATGGGCTATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Z4JIQ6