Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF-MazE
Location 1306930..1307507 Replicon chromosome
Accession NZ_AP018203
Organism Leptolyngbya boryana NIES-2135

Toxin (Protein)


Gene name mazE Uniprot ID A0A1Z4JC99
Locus tag CA740_RS05995 Protein ID WP_017285931.1
Coordinates 1306930..1307256 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazF Uniprot ID A0A1Z4JCF0
Locus tag CA740_RS06000 Protein ID WP_017285932.1
Coordinates 1307244..1307507 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA740_RS05960 1302114..1302488 - 375 WP_017285924.1 hypothetical protein -
CA740_RS05965 1302577..1302846 - 270 WP_017285925.1 hypothetical protein -
CA740_RS05970 1302906..1303244 - 339 WP_017285926.1 hypothetical protein -
CA740_RS05975 1303647..1304924 + 1278 WP_017285927.1 adenosylhomocysteinase -
CA740_RS05980 1304974..1305318 - 345 WP_017285928.1 DUF86 domain-containing protein -
CA740_RS05985 1305308..1305643 - 336 WP_017285929.1 nucleotidyltransferase family protein -
CA740_RS05990 1305741..1306160 + 420 Protein_1181 hypothetical protein -
CA740_RS05995 1306930..1307256 - 327 WP_017285931.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CA740_RS06000 1307244..1307507 - 264 WP_017285932.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CA740_RS06005 1307632..1308432 + 801 WP_017285933.1 mechanosensitive ion channel family protein -
CA740_RS06010 1308784..1309629 + 846 WP_017285934.1 3-deoxy-7-phosphoheptulonate synthase -
CA740_RS06015 1310086..1311828 - 1743 WP_017285935.1 fibronectin-binding domain-containing protein -
CA740_RS06020 1311889..1312134 + 246 WP_017285936.1 DUF370 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-101)

Antitoxin

SpoVT-AbrB domain

(12-49)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12201.35 Da        Isoelectric Point: 8.5508

>T31717 WP_017285931.1 NZ_AP018203:c1307256-1306930 [Leptolyngbya boryana NIES-2135]
MAMVVRRFDVFLVQLDPTVGSEIQKTRPCIIVSPDEMNRLLKTVLIAPLTSVRRNFPFRVDCQFQGKAGQIVLDQIRAVD
KTRLVQFLGQIGEEQRALLDTIADLFAA

Download         Length: 327 bp

>T31717 NZ_AP018203:c1307256-1306930 [Leptolyngbya boryana NIES-2135]
ATGGCAATGGTAGTTCGTCGTTTTGATGTGTTTCTTGTTCAGCTAGATCCAACTGTTGGATCTGAAATTCAGAAGACCCG
TCCTTGTATAATCGTCTCTCCCGATGAGATGAATCGTTTACTCAAAACGGTTTTGATAGCTCCATTAACTTCTGTAAGAC
GAAACTTCCCATTTCGAGTTGATTGTCAGTTTCAAGGCAAAGCGGGTCAGATTGTCCTAGATCAAATTCGAGCAGTAGAT
AAAACCCGACTTGTTCAATTTCTGGGTCAAATTGGCGAGGAGCAAAGAGCGCTTCTCGATACGATAGCTGACCTCTTTGC
TGCCTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9789.91 Da        Isoelectric Point: 4.2815

>AT31717 WP_017285932.1 NZ_AP018203:c1307507-1307244 [Leptolyngbya boryana NIES-2135]
MSGTIRTRLVKFGNSQGIRIPKLLLEQSGIQSEVEIEVEGNRLIIRPVSHPRAGWEAAIDAEIAKSGDEGLLDDETSTQW
DETEWQW

Download         Length: 264 bp

>AT31717 NZ_AP018203:c1307507-1307244 [Leptolyngbya boryana NIES-2135]
ATGAGTGGAACAATTCGGACTCGACTTGTCAAATTTGGTAATTCTCAAGGAATTCGGATTCCGAAGCTGTTACTAGAGCA
AAGTGGCATTCAAAGTGAAGTTGAAATCGAAGTCGAAGGCAATCGCTTGATTATTCGTCCTGTATCTCATCCTCGTGCTG
GATGGGAAGCAGCGATCGATGCAGAAATCGCAAAATCTGGGGATGAAGGCTTGCTGGATGATGAAACCTCAACTCAATGG
GACGAAACCGAATGGCAATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Z4JC99

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z4JCF0

References