Detailed information of TA system TA031712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 4426647..4427313
• Replicon: chromosome
• Accession: NZ_AP018164
• Organism: Mycobacterium shigaense strain JCM 32072

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MSG_RS20610
• Protein ID: WP_096442529.1
• Coordinates: 4426647..4427120 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: MSG_RS20615
• Protein ID: WP_096442531.1
• Coordinates: 4427125..4427313 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSG_RS20590	4422932..4424158	+	1227	WP_096442521.1	acetyl-CoA acetyltransferase	-
MSG_RS20595	4424161..4425675	+	1515	WP_096442523.1	carotenoid oxygenase family protein	-
MSG_RS20600	4425672..4426151	+	480	WP_096442525.1	VOC family protein	-
MSG_RS20605	4426155..4426589	-	435	WP_096442527.1	hypothetical protein	-
MSG_RS20610	4426647..4427120	-	474	WP_096442529.1	SRPBCC family protein	Toxin
MSG_RS20615	4427125..4427313	-	189	WP_096442531.1	antitoxin	Antitoxin
MSG_RS20620	4427463..4429856	-	2394	WP_096442533.1	cation-translocating P-type ATPase	-
MSG_RS20625	4429853..4431436	-	1584	WP_096442535.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17165.94 Da
• Isoelectric Point: 10.0646

>T31712 WP_096442529.1 NZ_AP018164:c4427120-4426647 [Mycobacterium shigaense]
MAKLSGSIDVPLPPHQAWQHASDLSRYKDWLSIHRVWRSKLPDVLEKGTVIESIVEVKGMLNRIKWTIVHYKPPEAMTLN
GDGVGGVKVKLMAKVKQKEDGSVVSFDVHLGGPALFGPIGMIVAAALKGDINQSLEQFVTVFTSPDPGMNGDRGARR

Nucleotide

• Length: 474 bp

>T31712 NZ_AP018164:c4427120-4426647 [Mycobacterium shigaense]
ATGGCCAAACTCTCCGGATCCATCGACGTACCGCTGCCGCCCCACCAGGCGTGGCAGCATGCCTCTGACCTGTCCCGCTA
CAAGGACTGGCTGAGCATCCACCGCGTGTGGCGCAGCAAATTGCCCGACGTGCTGGAGAAGGGCACGGTGATCGAATCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGATCAAGTGGACGATCGTGCACTACAAGCCGCCGGAGGCGATGACGCTCAAC
GGCGACGGTGTGGGCGGGGTCAAGGTCAAGCTGATGGCCAAGGTCAAGCAGAAGGAAGACGGCTCTGTGGTCAGCTTCGA
CGTGCACCTCGGCGGCCCGGCGCTGTTCGGGCCGATCGGCATGATCGTCGCGGCCGCGCTGAAGGGCGACATCAATCAGT
CACTGGAGCAGTTCGTGACGGTGTTCACCTCTCCCGATCCCGGCATGAATGGCGACCGCGGCGCGCGGCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6671.33 Da
• Isoelectric Point: 4.7027

>AT31712 WP_096442531.1 NZ_AP018164:c4427313-4427125 [Mycobacterium shigaense]
MGLLDKAKDLLAQNADKVGTAIDKAGEFVDDKTQGKYSDTIHQVQEQAKKAAEAAAKDDDHN

Nucleotide

• Length: 189 bp

>AT31712 NZ_AP018164:c4427313-4427125 [Mycobacterium shigaense]
ATGGGATTGCTGGACAAAGCGAAGGACTTGCTGGCGCAGAATGCCGACAAGGTGGGGACGGCGATCGACAAGGCGGGCGA
GTTCGTCGACGACAAGACGCAGGGCAAGTACTCCGACACCATTCACCAGGTGCAGGAACAAGCCAAGAAAGCCGCCGAGG
CGGCGGCCAAGGACGACGACCACAACTGA