Detailed information of TA system TA031711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09812-MazE
• Location: 2690664..2691298
• Replicon: chromosome
• Accession: NZ_AP018150
• Organism: Mycoavidus cysteinexigens strain B1EB

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A2Z6EYT2
• Locus tag: MCB1EB_RS11375
• Protein ID: WP_045364155.1
• Coordinates: 2690664..2691029 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MCB1EB_RS11380
• Protein ID: WP_197721979.1
• Coordinates: 2691026..2691298 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCB1EB_RS11370	2686074..2690456	+	4383	WP_126354035.1	NACHT domain-containing protein	-
MCB1EB_RS11375	2690664..2691029	-	366	WP_045364155.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MCB1EB_RS11380	2691026..2691298	-	273	WP_197721979.1	hypothetical protein	Antitoxin
MCB1EB_RS11385	2691489..2694434	-	2946	WP_045364151.1	Tn3 family transposase	-
MCB1EB_RS11390	2694605..2694922	+	318	Protein_2243	transposase	-
MCB1EB_RS11395	2694956..2695096	-	141	Protein_2244	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2694950..2695096	146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-114)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13278.07 Da
• Isoelectric Point: 6.4784

>T31711 WP_045364155.1 NZ_AP018150:c2691029-2690664 [Mycoavidus cysteinexigens]
MNRRKVAQPGDVYWIDPNPVAGREMKDRHRFVVITPPEINALGVSMTVPITSGGQFARDNGLTVVISGHDTNGVAVCNHV
RSFDLNAREKVGTARYIETLDQVTTNEIISHVLSIIDPEIG

Nucleotide

• Length: 366 bp

>T31711 NZ_AP018150:c2691029-2690664 [Mycoavidus cysteinexigens]
ATGAACCGACGCAAAGTGGCTCAGCCTGGCGATGTGTACTGGATCGATCCAAATCCAGTGGCCGGACGAGAAATGAAAGA
CAGGCACCGTTTCGTTGTGATCACTCCGCCAGAAATTAATGCGTTGGGCGTATCCATGACAGTACCTATTACCAGTGGTG
GACAATTTGCCCGAGATAATGGATTAACAGTTGTCATTTCAGGACATGATACAAATGGCGTGGCTGTATGCAATCATGTT
AGGTCATTTGATCTCAATGCTCGTGAAAAAGTCGGTACCGCTCGCTATATTGAGACTTTGGATCAAGTCACGACCAATGA
AATTATCTCGCATGTTTTGAGTATTATTGATCCTGAGATAGGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10011.78 Da
• Isoelectric Point: 8.0460

>AT31711 WP_197721979.1 NZ_AP018150:c2691298-2691026 [Mycoavidus cysteinexigens]
MITVNIRKQGGAAVMTIPASVLKMLNVEAGAQLEIDVVQGAFTAKPVTKHLRKRYSLHELLRGVTPEQMVILNEQIEWAR
EGEPMGRECL

Nucleotide

• Length: 273 bp

>AT31711 NZ_AP018150:c2691298-2691026 [Mycoavidus cysteinexigens]
ATGATTACTGTTAACATCCGCAAACAAGGTGGCGCAGCAGTGATGACTATCCCTGCTAGTGTGTTAAAAATGCTTAACGT
TGAAGCTGGCGCTCAGCTAGAAATTGATGTCGTTCAAGGTGCATTTACCGCCAAACCCGTCACAAAACATTTGCGTAAGC
GTTATTCCTTGCACGAATTGCTACGAGGGGTCACCCCAGAGCAAATGGTTATACTGAACGAACAAATCGAATGGGCTCGT
GAGGGCGAACCCATGGGTCGGGAATGTTTATGA

Structures

Toxin

• AlphaFold DB: A0A2Z6EYT2