Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 2084248..2084780 Replicon chromosome
Accession NZ_AP018150
Organism Mycoavidus cysteinexigens strain B1EB

Toxin (Protein)


Gene name mazF Uniprot ID A0A2Z6EWR2
Locus tag MCB1EB_RS08865 Protein ID WP_045365061.1
Coordinates 2084457..2084780 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2Z6EWX4
Locus tag MCB1EB_RS08860 Protein ID WP_045365064.1
Coordinates 2084248..2084460 (+) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCB1EB_RS08855 2082384..2083472 - 1089 WP_045365067.1 DUF1016 family protein -
MCB1EB_RS08860 2084248..2084460 + 213 WP_045365064.1 antitoxin MazE family protein Antitoxin
MCB1EB_RS08865 2084457..2084780 + 324 WP_045365061.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MCB1EB_RS08875 2085052..2085432 - 381 WP_174232251.1 MerR family transcriptional regulator -
MCB1EB_RS08880 2085454..2085867 - 414 WP_081678036.1 integration host factor subunit alpha -
MCB1EB_RS08885 2085925..2088354 - 2430 WP_045365059.1 phenylalanine--tRNA ligase subunit beta -
MCB1EB_RS08890 2088387..2089400 - 1014 WP_026921278.1 phenylalanine--tRNA ligase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

Antitoxin MazE-like

(7-69)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11652.55 Da        Isoelectric Point: 10.4734

>T31710 WP_045365061.1 NZ_AP018150:2084457-2084780 [Mycoavidus cysteinexigens]
VKRGDLATIALQGAYGKPHPALIIQSDLFNEHPSVTVLPVTSELRDPSVFRLAVTPSKTNGLQKESQIMIDKVQTVPREK
VGLSFGRLDKQTLLSVNRALAVFLGFA

Download         Length: 324 bp

>T31710 NZ_AP018150:2084457-2084780 [Mycoavidus cysteinexigens]
GTGAAGCGTGGAGATTTGGCAACAATTGCTTTGCAAGGCGCTTATGGCAAACCGCACCCTGCACTGATTATTCAATCTGA
TCTCTTTAATGAGCATCCATCCGTTACTGTACTACCTGTAACGAGTGAGCTACGAGATCCATCAGTGTTTCGCTTGGCGG
TAACTCCTTCTAAAACTAATGGCTTGCAAAAAGAATCGCAGATCATGATTGATAAAGTGCAGACAGTTCCGCGAGAAAAA
GTAGGACTTTCATTTGGCCGCTTGGATAAACAAACGCTGCTGTCTGTGAATCGTGCTTTAGCTGTCTTCCTTGGTTTTGC
ATAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 8400.38 Da        Isoelectric Point: 7.2797

>AT31710 WP_045365064.1 NZ_AP018150:2084248-2084460 [Mycoavidus cysteinexigens]
MENSISHRIKKHRDELRAAGFRPIQIWVPDTRRQDFTDKCRQQSLALRNDPHETEILNWLQEASDTEGWK

Download         Length: 213 bp

>AT31710 NZ_AP018150:2084248-2084460 [Mycoavidus cysteinexigens]
ATGGAAAATAGTATCTCTCACCGTATAAAAAAGCATCGTGACGAATTACGTGCAGCAGGTTTTCGCCCAATACAAATTTG
GGTACCAGATACTCGCCGCCAAGATTTTACGGATAAGTGCCGCCAACAATCATTGGCGTTGAGAAATGATCCACACGAAA
CCGAAATACTAAACTGGCTGCAAGAGGCTTCTGATACAGAGGGCTGGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6EWR2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z6EWX4

References