Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpA-brnA/BrnT_toxin-BrnA
Location 1625275..1625863 Replicon chromosome
Accession NZ_AP018150
Organism Mycoavidus cysteinexigens strain B1EB

Toxin (Protein)


Gene name shpA Uniprot ID A0A2Z6EVP4
Locus tag MCB1EB_RS06785 Protein ID WP_045361892.1
Coordinates 1625275..1625541 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A2Z6EVK4
Locus tag MCB1EB_RS06790 Protein ID WP_045361888.1
Coordinates 1625528..1625863 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCB1EB_RS06775 1621259..1623844 - 2586 WP_126353931.1 hypothetical protein -
MCB1EB_RS06780 1623887..1624660 - 774 WP_126353932.1 hypothetical protein -
MCB1EB_RS06785 1625275..1625541 + 267 WP_045361892.1 BrnT family toxin Toxin
MCB1EB_RS06790 1625528..1625863 + 336 WP_045361888.1 BrnA antitoxin family protein Antitoxin
MCB1EB_RS06795 1626122..1626415 - 294 WP_126353933.1 hypothetical protein -
MCB1EB_RS06800 1626688..1627464 + 777 WP_126353934.1 hypothetical protein -
MCB1EB_RS06805 1627579..1630302 - 2724 WP_052393580.1 pentapeptide repeat-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1584194..1626415 42221


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(28-104)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10440.60 Da        Isoelectric Point: 4.7341

>T31707 WP_045361892.1 NZ_AP018150:1625275-1625541 [Mycoavidus cysteinexigens]
MDYEFDPAKDESNLDKHGLSLADAENFEWETAVVYEDTRQQYAERRFEVTGYIGERLHVMVYCLRGDAYRIISLRKANPR
EVKRYAET

Download         Length: 267 bp

>T31707 NZ_AP018150:1625275-1625541 [Mycoavidus cysteinexigens]
ATGGATTACGAATTTGACCCAGCCAAAGACGAAAGCAATCTTGATAAACACGGTTTGTCGCTGGCTGACGCTGAGAACTT
TGAATGGGAAACAGCCGTAGTCTATGAAGACACTCGCCAGCAATATGCCGAGCGTCGGTTTGAAGTAACCGGCTATATCG
GCGAGCGCTTGCATGTGATGGTCTACTGCCTGCGAGGTGATGCTTATCGCATCATCAGTTTGCGCAAGGCCAACCCGAGA
GAGGTAAAACGTTATGCCGAAACTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12273.13 Da        Isoelectric Point: 9.5387

>AT31707 WP_045361888.1 NZ_AP018150:1625528-1625863 [Mycoavidus cysteinexigens]
MPKLKPGTVIPTPDEEVIINAGIAADPDSRELDDEWFAKAKPANKVLPPEIYSALVAKRPRGRPKADETKVFTAIRLDAD
LLDKFKATGKGWQTRVNAALRQYLAEHPLAQ

Download         Length: 336 bp

>AT31707 NZ_AP018150:1625528-1625863 [Mycoavidus cysteinexigens]
ATGCCGAAACTTAAACCTGGGACAGTTATCCCTACACCAGATGAAGAAGTTATCATCAACGCAGGCATTGCTGCTGACCC
TGATAGCCGTGAGCTTGATGATGAGTGGTTCGCTAAAGCCAAGCCCGCAAATAAAGTGCTCCCGCCTGAAATATACTCCG
CGTTAGTTGCCAAGCGCCCTCGCGGGCGCCCGAAAGCTGATGAAACCAAAGTCTTTACTGCGATTCGCTTAGATGCCGAC
TTGTTAGATAAATTCAAGGCAACTGGTAAGGGCTGGCAAACCCGCGTGAATGCTGCTTTGCGCCAGTACTTGGCCGAGCA
CCCGCTGGCTCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6EVP4

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z6EVK4

References