Detailed information of TA system TA031706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 491194..491804
• Replicon: chromosome
• Accession: NZ_AP018150
• Organism: Mycoavidus cysteinexigens strain B1EB

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MCB1EB_RS02115
• Protein ID: WP_045365925.1
• Coordinates: 491194..491499 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2Z6ET34
• Locus tag: MCB1EB_RS02120
• Protein ID: WP_045363236.1
• Coordinates: 491496..491804 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCB1EB_RS02085	486246..487211	-	966	WP_045363251.1	hypothetical protein	-
MCB1EB_RS02090	487208..487861	-	654	WP_045363249.1	DUF2612 domain-containing protein	-
MCB1EB_RS02095	487874..489019	-	1146	WP_045363247.1	baseplate J/gp47 family protein	-
MCB1EB_RS02100	489279..489626	-	348	WP_045363243.1	hypothetical protein	-
MCB1EB_RS02105	489623..490321	-	699	WP_045363240.1	hypothetical protein	-
MCB1EB_RS02110	490318..491148	-	831	WP_045363238.1	hypothetical protein	-
MCB1EB_RS02115	491194..491499	+	306	WP_045365925.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MCB1EB_RS02120	491496..491804	+	309	WP_045363236.1	putative addiction module antidote protein	Antitoxin
MCB1EB_RS02125	491796..492119	-	324	WP_045363234.1	hypothetical protein	-
MCB1EB_RS02130	492123..492863	-	741	WP_045363232.1	hypothetical protein	-
MCB1EB_RS02135	492860..494524	-	1665	WP_045363230.1	phage tail tape measure protein	-
MCB1EB_RS02145	494672..495067	-	396	WP_045363228.1	hypothetical protein	-
MCB1EB_RS02150	495064..495504	-	441	WP_045363225.1	DUF3277 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	467798..517279	49481

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-95)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11684.43 Da
• Isoelectric Point: 10.1231

>T31706 WP_045365925.1 NZ_AP018150:491194-491499 [Mycoavidus cysteinexigens]
MYTILRSEEFDVWLHTLRDLRGKARILGRIKSAENGNFGNSEPVGEGVSEMKIDFGPGYRVYYTRHELVVYVLLLGGDKS
TQKRDIKHARRMALDLKRSKE

Nucleotide

• Length: 306 bp

>T31706 NZ_AP018150:491194-491499 [Mycoavidus cysteinexigens]
ATGTACACTATTTTGCGTTCTGAAGAATTCGATGTCTGGCTTCATACTCTGCGGGATTTGCGTGGTAAGGCCAGAATTTT
GGGCCGGATCAAATCCGCTGAAAATGGAAATTTTGGGAACTCGGAACCTGTTGGCGAAGGCGTTTCGGAGATGAAAATCG
ACTTCGGGCCAGGTTATAGGGTGTACTACACACGGCATGAGTTAGTGGTTTATGTTTTGCTTCTAGGCGGAGATAAATCG
ACCCAAAAGCGGGATATTAAGCATGCTAGGCGGATGGCCTTGGACCTTAAGAGGAGCAAAGAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11254.73 Da
• Isoelectric Point: 4.8018

>AT31706 WP_045363236.1 NZ_AP018150:491496-491804 [Mycoavidus cysteinexigens]
MTQYAKFDASDYLDNEETIAEYLSAALEDPNPDVFLAALSDVAKARGITYIAKKAGLGRESLYKTLTPGSKPRYDTIRKL
IDALDIKLSISATMNDGYRLSN

Nucleotide

• Length: 309 bp

>AT31706 NZ_AP018150:491496-491804 [Mycoavidus cysteinexigens]
ATGACTCAATATGCAAAATTCGACGCATCGGATTATCTCGATAATGAAGAGACGATTGCAGAATATTTAAGTGCTGCATT
AGAAGACCCCAATCCGGATGTTTTCCTTGCTGCATTGAGCGATGTCGCCAAAGCACGTGGCATAACGTATATAGCGAAGA
AAGCCGGTCTTGGGCGAGAAAGTCTCTACAAAACGCTAACGCCTGGCTCAAAGCCTCGCTACGACACGATCCGTAAATTG
ATTGATGCCCTCGATATTAAGCTCTCTATTTCAGCCACGATGAATGATGGGTATCGGTTATCAAACTAA

Structures

Antitoxin

• AlphaFold DB: A0A2Z6ET34