Detailed information of TA system TA031704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2327187..2327828
• Replicon: chromosome
• Accession: NZ_AP018112
• Organism: Burkholderia stabilis strain FERMP-21014

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BSFP_RS27570
• Protein ID: WP_047901477.1
• Coordinates: 2327187..2327525 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1J9PWC2
• Locus tag: BSFP_RS27575
• Protein ID: WP_039344756.1
• Coordinates: 2327529..2327828 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSFP_RS27555	2323754..2325187	-	1434	WP_096474907.1	aldehyde dehydrogenase family protein	-
BSFP_RS27560	2325217..2326857	-	1641	WP_096474908.1	acetolactate synthase large subunit	-
BSFP_RS27570	2327187..2327525	+	339	WP_047901477.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BSFP_RS27575	2327529..2327828	+	300	WP_039344756.1	putative addiction module antidote protein	Antitoxin
BSFP_RS27580	2328202..2328489	+	288	WP_197706765.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BSFP_RS27585	2328502..2328804	+	303	WP_096474911.1	HigA family addiction module antidote protein	-
BSFP_RS27590	2328838..2330124	-	1287	WP_096474912.1	DUF445 domain-containing protein	-
BSFP_RS27595	2330336..2331556	-	1221	WP_096474913.1	FAD-dependent oxidoreductase	-
BSFP_RS27600	2331546..2331872	-	327	WP_096474914.1	non-heme iron oxygenase ferredoxin subunit	-
BSFP_RS27605	2331890..2332366	-	477	WP_014900133.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (26-98)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12625.51 Da
• Isoelectric Point: 8.9480

>T31704 WP_047901477.1 NZ_AP018112:2327187-2327525 [Burkholderia stabilis]
MVDILQSMPYSAPTFSIRTTDVFDAWFAGLQDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLCGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T31704 NZ_AP018112:2327187-2327525 [Burkholderia stabilis]
ATGGTTGACATACTGCAATCAATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTT
TGCCGGCCTGCAGGATCGCGTCGCGAAGCGCCGCATCCAGGCGCGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCCGGCTCGCCGGTCGTCGAAATGCGCATCGACCATGGCCCCGGTTATCGCGTCTACTACGTCCGGCGCGGA
ACGATCTGGGTCATCCTGCTCTGCGGCGGCGATAAATCGACGCAACAGGCCGACATTCGCGCCGCGCACGCGATGCTTGC
GCATCTCGACATGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10649.01 Da
• Isoelectric Point: 6.3217

>AT31704 WP_039344756.1 NZ_AP018112:2327529-2327828 [Burkholderia stabilis]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGSKAHA

Nucleotide

• Length: 300 bp

>AT31704 NZ_AP018112:2327529-2327828 [Burkholderia stabilis]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAAACCGAAGACGACATGGCCGACTACTTCGAGGC
GTGCCTGCAAGAGGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGCCTGTCGCGCGAAGGACTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCTCGAAAGCGCACGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1J9PWC2