Detailed information of TA system TA031703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1616839..1617472
• Replicon: chromosome
• Accession: NZ_AP018112
• Organism: Burkholderia stabilis strain FERMP-21014

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BSFP_RS24355
• Protein ID: WP_096474391.1
• Coordinates: 1616839..1617252 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BSFP_RS24360
• Protein ID: WP_096474392.1
• Coordinates: 1617239..1617472 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSFP_RS24335	1612669..1613976	+	1308	WP_096474388.1	shikimate transporter	-
BSFP_RS24340	1614180..1615481	+	1302	WP_047903294.1	UDP-N-acetylglucosamine 1-carboxyvinyltransferase	-
BSFP_RS24345	1615610..1616221	-	612	WP_096474389.1	hypothetical protein	-
BSFP_RS24350	1616401..1616796	+	396	WP_096474390.1	DUF4440 domain-containing protein	-
BSFP_RS24355	1616839..1617252	-	414	WP_096474391.1	PIN domain-containing protein	Toxin
BSFP_RS24360	1617239..1617472	-	234	WP_096474392.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BSFP_RS24365	1617633..1618403	+	771	WP_096474393.1	helix-turn-helix domain-containing protein	-
BSFP_RS24370	1618501..1620801	-	2301	WP_096474394.1	ATP-dependent DNA helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-119)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15225.38 Da
• Isoelectric Point: 5.1973

>T31703 WP_096474391.1 NZ_AP018112:c1617252-1616839 [Burkholderia stabilis]
MSGVESAKSFVDSNVVLYLLSEDETKANRAEALFLRRPTISVQVLNEVASVCSRKLRMPWSDVGQFLEPIRSLCHVVPVT
IDTHDLARRLAERHGLSFYDACIVAAASLEGCEYLYTEDMHDGLRVDGGPVLRNPFA

Nucleotide

• Length: 414 bp

>T31703 NZ_AP018112:c1617252-1616839 [Burkholderia stabilis]
ATGAGCGGGGTTGAGTCGGCGAAATCGTTCGTCGACAGCAATGTCGTGCTTTATCTGCTGTCCGAGGACGAAACCAAGGC
GAATCGTGCGGAGGCACTGTTCCTTCGGCGGCCCACCATCAGTGTCCAGGTACTGAACGAGGTCGCCAGCGTTTGCAGCC
GAAAATTGCGGATGCCGTGGAGCGACGTGGGGCAGTTTCTGGAACCGATCAGGAGCTTGTGCCATGTCGTACCGGTCACG
ATCGATACGCATGACCTGGCGCGAAGGCTGGCGGAACGGCATGGCTTGTCCTTCTATGACGCGTGCATCGTCGCCGCCGC
GTCGCTCGAGGGCTGCGAGTACCTGTACACCGAGGACATGCACGACGGCCTGCGCGTGGACGGCGGTCCGGTGCTGCGAA
ATCCGTTTGCCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8750.99 Da
• Isoelectric Point: 10.2843

>AT31703 WP_096474392.1 NZ_AP018112:c1617472-1617239 [Burkholderia stabilis]
MQVAKWGNSLAVRLPASVVDALELREGDDIEIVVDSPRVFAVSRKPRADELLQRLRRFRGKLPADFKFSRDDANERG

Nucleotide

• Length: 234 bp

>AT31703 NZ_AP018112:c1617472-1617239 [Burkholderia stabilis]
ATGCAGGTCGCAAAGTGGGGCAATAGCCTGGCCGTCCGCCTTCCCGCCAGTGTCGTCGATGCACTGGAGCTCCGCGAAGG
GGACGATATCGAAATCGTCGTCGATAGTCCGCGCGTTTTTGCCGTCAGCCGCAAGCCGAGGGCCGATGAACTTCTGCAAC
GGTTGCGGCGTTTCAGGGGCAAGCTGCCCGCCGATTTCAAGTTCAGCCGGGACGACGCGAATGAGCGGGGTTGA