Detailed information of TA system TA031694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 456444..457120
• Replicon: chromosome
• Accession: NZ_AP018111
• Organism: Burkholderia stabilis strain FERMP-21014

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A0J5X1U9
• Locus tag: BSFP_RS01880
• Protein ID: WP_027787436.1
• Coordinates: 456704..457120 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: BSFP_RS01875
• Protein ID: WP_096473010.1
• Coordinates: 456444..456707 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSFP_RS01860	451989..454274	+	2286	WP_096470891.1	TonB-dependent siderophore receptor	-
BSFP_RS01865	454361..455200	+	840	WP_096470892.1	N(5)-hydroxyornithine transformylase PvdF	-
BSFP_RS01870	455200..456213	+	1014	WP_096473009.1	acetyltransferase	-
BSFP_RS01875	456444..456707	+	264	WP_096473010.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BSFP_RS01880	456704..457120	+	417	WP_027787436.1	PIN domain-containing protein	Toxin
BSFP_RS01885	457169..458074	-	906	WP_096470893.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
BSFP_RS01890	458043..459116	-	1074	WP_096473011.1	hypothetical protein	-
BSFP_RS01895	459459..460622	+	1164	WP_096470894.1	amidohydrolase	-
BSFP_RS01900	460808..461347	+	540	WP_096470895.1	outer membrane protein assembly factor BamE	-
BSFP_RS01905	461468..461713	+	246	WP_096470896.1	type II toxin-antitoxin system ParD family antitoxin	-
BSFP_RS01910	461703..462002	+	300	WP_096470897.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14536.66 Da
• Isoelectric Point: 7.8895

>T31694 WP_027787436.1 NZ_AP018111:456704-457120 [Burkholderia stabilis]
VTTLYMLDTNICSFIMRERPPVVLSRLQSCVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVSRLDGVLSWDTA
AVDATAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNGREFGRVAGLSLEDWAA

Nucleotide

• Length: 417 bp

>T31694 NZ_AP018111:456704-457120 [Burkholderia stabilis]
GTGACAACGCTCTATATGCTCGATACGAACATTTGTTCGTTCATCATGCGCGAGCGACCGCCGGTGGTGCTGTCGCGGCT
GCAGTCGTGCGTCAACGCGCAGCACCGGATCGTCGTGTCGGCGATTACCTATGCCGAGATGCGGTTCGGGGCCGTGGGCA
GGAAGGCGTCGCCGAAGCATGCGGAACTCGTGACAGCCTTTGTTTCGCGACTGGATGGCGTGCTGTCGTGGGATACGGCG
GCCGTCGATGCAACGGCCGCGATTCGCGCGGATCTCGCCGCGCGCGGCACGCCGATCGGCGCGAACGATGCGTCGATCGC
CGGGCATGCGATCGCGGCCGGCGCGGTGCTCGTCACGAACAACGGGCGGGAATTCGGCCGTGTCGCCGGGTTGTCGCTCG
AAGACTGGGCGGCCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9732.97 Da
• Isoelectric Point: 4.4944

>AT31694 WP_096473010.1 NZ_AP018111:456444-456707 [Burkholderia stabilis]
MRTVSIFKNARNQAIRIPKDMEFEGVTELEIRREGDTLLLRPVRPTWLSFANEPLADAEFLAERPAVIESGRFDLSADAG
SPTESGQ

Nucleotide

• Length: 264 bp

>AT31694 NZ_AP018111:456444-456707 [Burkholderia stabilis]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGGGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACGCTGCTGCTGCGCCCCGTGCGCCCCACGTGGCTCTCGTTCGCAAACGAAC
CGCTGGCCGACGCCGAGTTCCTCGCCGAACGCCCGGCCGTCATCGAGTCCGGCCGTTTCGACCTGTCCGCCGATGCCGGC
TCGCCGACGGAGTCCGGCCAGTGA

Structures

Toxin

• AlphaFold DB: A0A0J5X1U9