Detailed information of TA system TA031691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT-BrnA
• Location: 2227897..2228422
• Replicon: chromosome
• Accession: NZ_AP018052
• Organism: Thiohalobacter thiocyanaticus strain FOKN1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: CFK21_RS10360
• Protein ID: WP_096366585.1
• Coordinates: 2228138..2228422 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: CFK21_RS10355
• Protein ID: WP_096366584.1
• Coordinates: 2227897..2228145 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFK21_RS10335	2224654..2225397	+	744	WP_096366581.1	peptidoglycan binding protein CsiV	-
CFK21_RS10340	2225622..2226017	-	396	WP_096366582.1	type II toxin-antitoxin system YafO family toxin	-
CFK21_RS10345	2226017..2226316	-	300	WP_096367583.1	type I toxin-antitoxin system antitoxin YafN	-
CFK21_RS15255	2226553..2226996	-	444	WP_157745607.1	VPLPA-CTERM sorting domain-containing protein	-
CFK21_RS10350	2227427..2227702	-	276	WP_096366583.1	GIY-YIG nuclease family protein	-
CFK21_RS10355	2227897..2228145	-	249	WP_096366584.1	BrnA antitoxin family protein	Antitoxin
CFK21_RS10360	2228138..2228422	-	285	WP_096366585.1	BrnT family toxin	Toxin
CFK21_RS10365	2228525..2230093	-	1569	WP_096366586.1	bifunctional GNAT family N-acetyltransferase/carbon-nitrogen hydrolase family protein	-
CFK21_RS10370	2230103..2230567	-	465	WP_096366587.1	peroxiredoxin	-
CFK21_RS10375	2230799..2231545	-	747	WP_096366588.1	S-methyl-5'-thioinosine phosphorylase	-
CFK21_RS10380	2231567..2232121	-	555	WP_096366589.1	hypoxanthine-guanine phosphoribosyltransferase	-
CFK21_RS10385	2232252..2233196	-	945	WP_096366590.1	patatin-like phospholipase RssA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (3-59)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10894.23 Da
• Isoelectric Point: 7.4655

>T31691 WP_096366585.1 NZ_AP018052:c2228422-2228138 [Thiohalobacter thiocyanaticus]
MDYEWDPRKAAHNLSKHGVDFADAVVALEDENALTIEDRDSGELRFKTLGMGPYLKVLYVVHAERGNNRIRIISARRATR
REIRQYYQGLDHHG

Nucleotide

• Length: 285 bp

>T31691 NZ_AP018052:c2228422-2228138 [Thiohalobacter thiocyanaticus]
ATGGACTATGAGTGGGATCCACGGAAGGCTGCCCATAACCTCAGCAAGCATGGCGTCGATTTCGCCGATGCAGTGGTTGC
GCTCGAAGATGAGAATGCGCTGACGATTGAGGATCGGGATAGCGGCGAACTGAGGTTCAAGACCCTGGGAATGGGGCCGT
ACCTGAAAGTCCTATATGTGGTGCATGCCGAGCGTGGGAATAATCGCATACGCATCATATCTGCCCGGCGTGCAACCAGA
CGTGAAATCAGACAGTACTATCAAGGTCTCGATCACCATGGCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9441.83 Da
• Isoelectric Point: 10.2563

>AT31691 WP_096366584.1 NZ_AP018052:c2228145-2227897 [Thiohalobacter thiocyanaticus]
MAEDFSKAKRGPVVKSDPNKVRITIRLDTDIVEHFKNRVHASGGGNYQTLINNALREYIQAHDKELESILRKIIREELKR
AV

Nucleotide

• Length: 249 bp

>AT31691 NZ_AP018052:c2228145-2227897 [Thiohalobacter thiocyanaticus]
ATGGCTGAAGATTTTTCCAAAGCGAAGCGCGGACCGGTCGTCAAATCCGATCCAAACAAGGTTCGGATCACAATACGGCT
CGATACGGATATTGTCGAACATTTCAAGAACCGGGTGCATGCAAGTGGTGGTGGAAATTATCAGACGCTGATTAACAATG
CCTTGAGGGAATATATACAGGCGCACGATAAGGAACTTGAAAGCATCTTGCGTAAAATAATACGGGAAGAATTGAAGCGC
GCTGTGTAA