Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3099107..3099677 Replicon chromosome
Accession NZ_AP018036
Organism Mycobacterium tuberculosis strain HN-506

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CPZ04_RS15250 Protein ID WP_003414166.1
Coordinates 3099107..3099463 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CPZ04_RS15255 Protein ID WP_003901465.1
Coordinates 3099447..3099677 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ04_RS15230 3094559..3096247 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CPZ04_RS15235 3096251..3096577 - 327 WP_003414157.1 hypothetical protein -
CPZ04_RS15240 3096750..3097337 + 588 WP_003914429.1 DUF3558 family protein -
CPZ04_RS15245 3097356..3099005 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
CPZ04_RS15250 3099107..3099463 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CPZ04_RS15255 3099447..3099677 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CPZ04_RS15260 3099720..3100763 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
CPZ04_RS15265 3100954..3101229 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CPZ04_RS21835 3101405..3101659 - 255 WP_003917684.1 hypothetical protein -
CPZ04_RS15275 3101807..3102211 + 405 WP_003414181.1 hypothetical protein -
CPZ04_RS15280 3102208..3102399 + 192 WP_003414184.1 hypothetical protein -
CPZ04_RS15285 3102463..3103752 + 1290 Protein_2890 transposase family protein -
CPZ04_RS15290 3103986..3104243 + 258 WP_003899489.1 hypothetical protein -
CPZ04_RS15295 3104348..3104659 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T31679 WP_003414166.1 NZ_AP018036:c3099463-3099107 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T31679 NZ_AP018036:c3099463-3099107 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT31679 WP_003901465.1 NZ_AP018036:c3099677-3099447 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT31679 NZ_AP018036:c3099677-3099447 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References