Detailed information of TA system TA031674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2859886..2860523
• Replicon: chromosome
• Accession: NZ_AP018036
• Organism: Mycobacterium tuberculosis strain HN-506

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: CPZ04_RS13905
• Protein ID: WP_003413180.1
• Coordinates: 2859886..2860281 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: CPZ04_RS13910
• Protein ID: WP_003413183.1
• Coordinates: 2860278..2860523 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ04_RS13860	2855290..2856501	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
CPZ04_RS13870	2856628..2857287	+	660	WP_003902296.1	hypothetical protein	-
CPZ04_RS13875	2857284..2857946	+	663	WP_003905878.1	hypothetical protein	-
CPZ04_RS13880	2857943..2858220	+	278	Protein_2621	type II toxin-antitoxin system VapB family antitoxin	-
CPZ04_RS13885	2858313..2858726	+	414	WP_003413164.1	PIN domain nuclease	-
CPZ04_RS13890	2858765..2859022	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
CPZ04_RS13895	2859019..2859396	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
CPZ04_RS13900	2859412..2859786	-	375	WP_003413177.1	hypothetical protein	-
CPZ04_RS13905	2859886..2860281	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
CPZ04_RS13910	2860278..2860523	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
CPZ04_RS13920	2860934..2861353	-	420	WP_003413190.1	A24 family peptidase	-
CPZ04_RS13925	2861365..2862174	-	810	WP_003413193.1	shikimate dehydrogenase	-
CPZ04_RS13930	2862171..2863424	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
CPZ04_RS13935	2863417..2863929	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T31674 WP_003413180.1 NZ_AP018036:c2860281-2859886 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T31674 NZ_AP018036:c2860281-2859886 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT31674 WP_003413183.1 NZ_AP018036:c2860523-2860278 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT31674 NZ_AP018036:c2860523-2860278 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31