Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2859886..2860523 Replicon chromosome
Accession NZ_AP018036
Organism Mycobacterium tuberculosis strain HN-506

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag CPZ04_RS13905 Protein ID WP_003413180.1
Coordinates 2859886..2860281 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag CPZ04_RS13910 Protein ID WP_003413183.1
Coordinates 2860278..2860523 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ04_RS13860 2855290..2856501 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CPZ04_RS13870 2856628..2857287 + 660 WP_003902296.1 hypothetical protein -
CPZ04_RS13875 2857284..2857946 + 663 WP_003905878.1 hypothetical protein -
CPZ04_RS13880 2857943..2858220 + 278 Protein_2621 type II toxin-antitoxin system VapB family antitoxin -
CPZ04_RS13885 2858313..2858726 + 414 WP_003413164.1 PIN domain nuclease -
CPZ04_RS13890 2858765..2859022 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
CPZ04_RS13895 2859019..2859396 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
CPZ04_RS13900 2859412..2859786 - 375 WP_003413177.1 hypothetical protein -
CPZ04_RS13905 2859886..2860281 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
CPZ04_RS13910 2860278..2860523 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
CPZ04_RS13920 2860934..2861353 - 420 WP_003413190.1 A24 family peptidase -
CPZ04_RS13925 2861365..2862174 - 810 WP_003413193.1 shikimate dehydrogenase -
CPZ04_RS13930 2862171..2863424 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
CPZ04_RS13935 2863417..2863929 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T31674 WP_003413180.1 NZ_AP018036:c2860281-2859886 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T31674 NZ_AP018036:c2860281-2859886 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT31674 WP_003413183.1 NZ_AP018036:c2860523-2860278 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT31674 NZ_AP018036:c2860523-2860278 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References