Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2248615..2249241 Replicon chromosome
Accession NZ_AP018036
Organism Mycobacterium tuberculosis strain HN-506

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag CPZ04_RS10875 Protein ID WP_003410075.1
Coordinates 2248843..2249241 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag CPZ04_RS10870 Protein ID WP_003911750.1
Coordinates 2248615..2248842 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ04_RS10860 2246654..2246998 - 345 WP_003410065.1 ferredoxin family protein -
CPZ04_RS10865 2247187..2248356 - 1170 WP_003899126.1 ATP-binding protein -
CPZ04_RS10870 2248615..2248842 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CPZ04_RS10875 2248843..2249241 + 399 WP_003410075.1 PIN domain nuclease Toxin
CPZ04_RS10880 2249424..2249855 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
CPZ04_RS10885 2249956..2250390 + 435 WP_003914358.1 DUF1398 domain-containing protein -
CPZ04_RS10900 2250827..2251006 - 180 Protein_2067 hypothetical protein -
CPZ04_RS10905 2251067..2251714 + 648 WP_071854215.1 transposase -
CPZ04_RS10910 2251668..2252258 + 591 WP_003899131.1 IS110 family transposase -
CPZ04_RS10915 2252386..2253642 - 1257 WP_003902247.1 HNH endonuclease -
CPZ04_RS10925 2253996..2254196 + 201 Protein_2071 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T31665 WP_003410075.1 NZ_AP018036:2248843-2249241 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T31665 NZ_AP018036:2248843-2249241 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT31665 WP_003911750.1 NZ_AP018036:2248615-2248842 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT31665 NZ_AP018036:2248615-2248842 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References