Detailed information of TA system TA031659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2180720..2181423
• Replicon: chromosome
• Accession: NZ_AP018036
• Organism: Mycobacterium tuberculosis strain HN-506

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: CPZ04_RS10445
• Protein ID: WP_003409778.1
• Coordinates: 2180720..2181049 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CPZ04_RS10450
• Protein ID: WP_003409780.1
• Coordinates: 2181046..2181423 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ04_RS10425	2177103..2178173	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
CPZ04_RS10430	2178170..2178685	+	516	WP_003409718.1	flavin reductase family protein	-
CPZ04_RS10435	2178682..2179743	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CPZ04_RS10440	2179740..2180510	+	771	WP_096362306.1	SDR family oxidoreductase	-
CPZ04_RS10445	2180720..2181049	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CPZ04_RS10450	2181046..2181423	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CPZ04_RS10455	2181420..2182010	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CPZ04_RS10460	2182065..2183429	+	1365	WP_003903691.1	HNH endonuclease	-
CPZ04_RS10465	2183584..2184036	-	453	WP_003899095.1	lipoprotein	-
CPZ04_RS10470	2184100..2184501	+	402	WP_003409869.1	hypothetical protein	-
CPZ04_RS10475	2184494..2184676	-	183	WP_003409870.1	hypothetical protein	-
CPZ04_RS10480	2184790..2185140	-	351	WP_003409871.1	hypothetical protein	-
CPZ04_RS10485	2185151..2186053	-	903	WP_003899097.1	hypothetical protein	-
CPZ04_RS10490	2186074..2186265	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T31659 WP_003409778.1 NZ_AP018036:c2181049-2180720 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T31659 NZ_AP018036:c2181049-2180720 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT31659 WP_003409780.1 NZ_AP018036:c2181423-2181046 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT31659 NZ_AP018036:c2181423-2181046 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8