Detailed information of TA system TA031650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1237592..1238160
• Replicon: chromosome
• Accession: NZ_AP018036
• Organism: Mycobacterium tuberculosis strain HN-506

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CPZ04_RS06080
• Protein ID: WP_003405865.1
• Coordinates: 1237786..1238160 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CPZ04_RS06075
• Protein ID: WP_003405863.1
• Coordinates: 1237592..1237789 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ04_RS06055	1233633..1234271	-	639	WP_003898730.1	lipid droplet-associated protein	-
CPZ04_RS06060	1234361..1235368	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CPZ04_RS06065	1235385..1236368	-	984	WP_003898731.1	hypothetical protein	-
CPZ04_RS06070	1236431..1237504	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CPZ04_RS06075	1237592..1237789	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CPZ04_RS06080	1237786..1238160	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CPZ04_RS06085	1238363..1239061	+	699	WP_003898733.1	hypothetical protein	-
CPZ04_RS06090	1239179..1239364	+	186	WP_003901093.1	hypothetical protein	-
CPZ04_RS06095	1239291..1239566	-	276	WP_031651777.1	hypothetical protein	-
CPZ04_RS06100	1239809..1240132	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CPZ04_RS06105	1240147..1240857	-	711	WP_003911399.1	hypothetical protein	-
CPZ04_RS21995	1240879..1241088	+	210	WP_003911400.1	hypothetical protein	-
CPZ04_RS06110	1241040..1241747	-	708	Protein_1159	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T31650 WP_003405865.1 NZ_AP018036:1237786-1238160 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T31650 NZ_AP018036:1237786-1238160 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT31650 WP_003405863.1 NZ_AP018036:1237592-1237789 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT31650 NZ_AP018036:1237592-1237789 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07