Detailed information of TA system TA031647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1012928..1013547
• Replicon: chromosome
• Accession: NZ_AP018036
• Organism: Mycobacterium tuberculosis strain HN-506

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: CPZ04_RS04935
• Protein ID: WP_003404726.1
• Coordinates: 1013113..1013547 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: CPZ04_RS04930
• Protein ID: WP_003898641.1
• Coordinates: 1012928..1013107 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ04_RS04920	1008401..1009981	+	1581	WP_003910977.1	serine hydrolase	-
CPZ04_RS04925	1009978..1012371	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
CPZ04_RS21735	1012644..1012802	+	159	WP_003404720.1	hypothetical protein	-
CPZ04_RS04930	1012928..1013107	+	180	WP_003898641.1	antitoxin	Antitoxin
CPZ04_RS04935	1013113..1013547	+	435	WP_003404726.1	SRPBCC family protein	Toxin
CPZ04_RS04940	1013645..1014418	+	774	WP_003404735.1	VOC family protein	-
CPZ04_RS04945	1014483..1014932	+	450	WP_003404738.1	hypothetical protein	-
CPZ04_RS04950	1015067..1015297	-	231	WP_003898642.1	hypothetical protein	-
CPZ04_RS04955	1015464..1016972	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
CPZ04_RS04960	1016974..1018212	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T31647 WP_003404726.1 NZ_AP018036:1013113-1013547 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T31647 NZ_AP018036:1013113-1013547 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT31647 WP_003898641.1 NZ_AP018036:1012928-1013107 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT31647 NZ_AP018036:1012928-1013107 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5