31619

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3110915..3111485 Replicon chromosome
Accession NZ_AP018035
Organism Mycobacterium tuberculosis strain HN-321

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CPZ12_RS15300 Protein ID WP_003414166.1
Coordinates 3110915..3111271 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CPZ12_RS15305 Protein ID WP_003901465.1
Coordinates 3111255..3111485 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ12_RS15280 3106365..3108053 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CPZ12_RS15285 3108057..3108383 - 327 WP_003414157.1 hypothetical protein -
CPZ12_RS15290 3108556..3109143 + 588 WP_003914429.1 DUF3558 family protein -
CPZ12_RS15295 3109162..3110813 + 1652 Protein_2892 CocE/NonD family hydrolase -
CPZ12_RS15300 3110915..3111271 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CPZ12_RS15305 3111255..3111485 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CPZ12_RS15310 3111528..3112571 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
CPZ12_RS15315 3112762..3113037 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CPZ12_RS21880 3113213..3113467 - 255 WP_003917684.1 hypothetical protein -
CPZ12_RS15325 3113615..3114019 + 405 WP_003414181.1 hypothetical protein -
CPZ12_RS15330 3114016..3114207 + 192 WP_003414184.1 hypothetical protein -
CPZ12_RS15335 3114271..3115560 + 1290 Protein_2900 transposase family protein -
CPZ12_RS15340 3115794..3116051 + 258 WP_003899489.1 hypothetical protein -
CPZ12_RS15345 3116156..3116467 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T31619 WP_003414166.1 NZ_AP018035:c3111271-3110915 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T31619 NZ_AP018035:c3111271-3110915 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT31619 WP_003901465.1 NZ_AP018035:c3111485-3111255 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT31619 NZ_AP018035:c3111485-3111255 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FBW3

References