Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1693329..1693945 Replicon chromosome
Accession NZ_AP018035
Organism Mycobacterium tuberculosis strain HN-321

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag CPZ12_RS08200 Protein ID WP_003407593.1
Coordinates 1693628..1693945 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag CPZ12_RS08195 Protein ID WP_003900349.1
Coordinates 1693329..1693631 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPZ12_RS08185 1689215..1691062 + 1848 WP_031666012.1 methylmalonyl-CoA mutase small subunit -
CPZ12_RS08190 1691063..1693315 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
CPZ12_RS08195 1693329..1693631 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
CPZ12_RS08200 1693628..1693945 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
CPZ12_RS08205 1693942..1694946 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
CPZ12_RS08210 1694999..1696288 + 1290 WP_003902090.1 serine hydrolase -
CPZ12_RS08215 1696361..1697134 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
CPZ12_RS08220 1697192..1697404 - 213 WP_003898900.1 dodecin family protein -
CPZ12_RS21815 1697414..1697638 - 225 WP_160522526.1 NUDIX domain-containing protein -
CPZ12_RS08230 1697908..1698936 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T31595 WP_003407593.1 NZ_AP018035:1693628-1693945 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T31595 NZ_AP018035:1693628-1693945 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT31595 WP_003900349.1 NZ_AP018035:1693329-1693631 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT31595 NZ_AP018035:1693329-1693631 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References