Detailed information of TA system TA031530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1233335..1233903
• Replicon: chromosome
• Accession: NZ_AP018034
• Organism: Mycobacterium tuberculosis strain HN-205

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: CPZ09_RS06050
• Protein ID: WP_003405865.1
• Coordinates: 1233529..1233903 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: CPZ09_RS06045
• Protein ID: WP_003405863.1
• Coordinates: 1233335..1233532 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ09_RS06025	1229376..1230014	-	639	WP_003898730.1	lipid droplet-associated protein	-
CPZ09_RS06030	1230104..1231111	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
CPZ09_RS06035	1231128..1232111	-	984	WP_003898731.1	hypothetical protein	-
CPZ09_RS06040	1232174..1233247	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
CPZ09_RS06045	1233335..1233532	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CPZ09_RS06050	1233529..1233903	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
CPZ09_RS06055	1234106..1234804	+	699	WP_003898733.1	hypothetical protein	-
CPZ09_RS06060	1234922..1235107	+	186	WP_003901093.1	hypothetical protein	-
CPZ09_RS06065	1235034..1235309	-	276	WP_003405867.1	hypothetical protein	-
CPZ09_RS06070	1235552..1235875	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
CPZ09_RS06075	1235890..1236600	-	711	WP_003911399.1	hypothetical protein	-
CPZ09_RS21955	1236622..1236831	+	210	WP_003911400.1	hypothetical protein	-
CPZ09_RS06080	1236783..1237490	-	708	Protein_1153	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T31530 WP_003405865.1 NZ_AP018034:1233529-1233903 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T31530 NZ_AP018034:1233529-1233903 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT31530 WP_003405863.1 NZ_AP018034:1233335-1233532 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT31530 NZ_AP018034:1233335-1233532 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07