Detailed information of TA system TA031483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2227258..2227946
• Replicon: chromosome
• Accession: NZ_AP018033
• Organism: Mycobacterium tuberculosis strain HN-024

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0A653
• Locus tag: CPZ07_RS10715
• Protein ID: WP_003409958.1
• Coordinates: 2227258..2227677 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: CPZ07_RS10720
• Protein ID: WP_003409968.1
• Coordinates: 2227686..2227946 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ07_RS10695	2222753..2223601	+	849	WP_003409942.1	class I SAM-dependent methyltransferase	-
CPZ07_RS10700	2223564..2225009	-	1446	WP_003409946.1	APC family permease	-
CPZ07_RS10705	2225188..2225874	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
CPZ07_RS10710	2226065..2227033	-	969	WP_003899116.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
CPZ07_RS10715	2227258..2227677	-	420	WP_003409958.1	type II toxin-antitoxin system VapC family toxin	Toxin
CPZ07_RS10720	2227686..2227946	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CPZ07_RS10725	2228089..2228445	+	357	Protein_2031	PE family protein	-
CPZ07_RS10730	2228592..2229152	-	561	WP_003410001.1	RES family NAD+ phosphorylase	-
CPZ07_RS10735	2229149..2229490	-	342	WP_003410003.1	DUF2384 domain-containing protein	-
CPZ07_RS10740	2229576..2229833	+	258	WP_003410006.1	hypothetical protein	-
CPZ07_RS10745	2229734..2230069	-	336	WP_003410009.1	dehydrogenase	-
CPZ07_RS10750	2230158..2230502	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
CPZ07_RS10755	2230496..2230744	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T31483 WP_003409958.1 NZ_AP018033:c2227677-2227258 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T31483 NZ_AP018033:c2227677-2227258 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT31483 WP_003409968.1 NZ_AP018033:c2227946-2227686 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT31483 NZ_AP018033:c2227946-2227686 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Toxin

• AlphaFold DB: A0A829C4H9

Antitoxin

• AlphaFold DB: A0A829C483