Detailed information of TA system TA031480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2191993..2192696
• Replicon: chromosome
• Accession: NZ_AP018033
• Organism: Mycobacterium tuberculosis strain HN-024

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: CPZ07_RS10455
• Protein ID: WP_003409778.1
• Coordinates: 2191993..2192322 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CPZ07_RS10460
• Protein ID: WP_003409780.1
• Coordinates: 2192319..2192696 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ07_RS10435	2188376..2189446	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
CPZ07_RS10440	2189443..2189958	+	516	WP_003409718.1	flavin reductase family protein	-
CPZ07_RS10445	2189955..2191016	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CPZ07_RS10450	2191013..2191783	+	771	WP_003409775.1	SDR family oxidoreductase	-
CPZ07_RS10455	2191993..2192322	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CPZ07_RS10460	2192319..2192696	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CPZ07_RS10465	2192693..2193283	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CPZ07_RS10470	2193338..2194702	+	1365	WP_003903691.1	HNH endonuclease	-
CPZ07_RS10475	2194857..2195309	-	453	WP_003899095.1	lipoprotein	-
CPZ07_RS10480	2195373..2195774	+	402	WP_003409869.1	hypothetical protein	-
CPZ07_RS10485	2195767..2195949	-	183	WP_003409870.1	hypothetical protein	-
CPZ07_RS10490	2196063..2196413	-	351	WP_003409871.1	hypothetical protein	-
CPZ07_RS10495	2196424..2197326	-	903	WP_003409874.1	hypothetical protein	-
CPZ07_RS10500	2197347..2197538	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T31480 WP_003409778.1 NZ_AP018033:c2192322-2191993 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T31480 NZ_AP018033:c2192322-2191993 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT31480 WP_003409780.1 NZ_AP018033:c2192696-2192319 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT31480 NZ_AP018033:c2192696-2192319 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8