Detailed information of TA system TA031467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1017389..1018008
• Replicon: chromosome
• Accession: NZ_AP018033
• Organism: Mycobacterium tuberculosis strain HN-024

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: CPZ07_RS04940
• Protein ID: WP_003404726.1
• Coordinates: 1017574..1018008 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: G0TFQ5
• Locus tag: CPZ07_RS04935
• Protein ID: WP_003404724.1
• Coordinates: 1017389..1017568 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPZ07_RS04925	1012862..1014442	+	1581	WP_003909313.1	serine hydrolase	-
CPZ07_RS04930	1014439..1016832	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
CPZ07_RS21550	1017105..1017263	+	159	WP_003404720.1	hypothetical protein	-
CPZ07_RS04935	1017389..1017568	+	180	WP_003404724.1	antitoxin	Antitoxin
CPZ07_RS04940	1017574..1018008	+	435	WP_003404726.1	SRPBCC family protein	Toxin
CPZ07_RS04945	1018106..1018879	+	774	WP_031661962.1	VOC family protein	-
CPZ07_RS04950	1018944..1019393	+	450	WP_003404738.1	hypothetical protein	-
CPZ07_RS04955	1019528..1019758	-	231	WP_003898642.1	hypothetical protein	-
CPZ07_RS04960	1019925..1021433	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
CPZ07_RS04965	1021435..1022674	-	1240	Protein_946	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T31467 WP_003404726.1 NZ_AP018033:1017574-1018008 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T31467 NZ_AP018033:1017574-1018008 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6394.06 Da
• Isoelectric Point: 4.3059

>AT31467 WP_003404724.1 NZ_AP018033:1017389-1017568 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLQDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT31467 NZ_AP018033:1017389-1017568 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCAGGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: G0TFQ5