Detailed information of TA system TA031436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prcAT/COG5642-COG5654
• Location: 2245424..2246364
• Replicon: chromosome
• Accession: NZ_AP017928
• Organism: Methylocaldum marinum strain S8

Toxin (Protein)

• Gene name: PrcT
• Uniprot ID: -
• Locus tag: sS8_RS09670
• Protein ID: WP_119629469.1
• Coordinates: 2245424..2245897 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: PrcA
• Uniprot ID: -
• Locus tag: sS8_RS09675
• Protein ID: WP_119629470.1
• Coordinates: 2245897..2246364 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
sS8_RS09650	2240834..2242198	-	1365	WP_119629465.1	lysine 2,3-aminomutase	-
sS8_RS09655	2242704..2243282	+	579	WP_119629466.1	MarR family transcriptional regulator	-
sS8_RS09660	2243370..2244110	+	741	WP_119629467.1	hypothetical protein	-
sS8_RS09665	2244249..2244848	+	600	WP_119629468.1	HdeD family acid-resistance protein	-
sS8_RS09670	2245424..2245897	-	474	WP_119629469.1	RES family NAD+ phosphorylase	Toxin
sS8_RS09675	2245897..2246364	-	468	WP_119629470.1	DUF2384 domain-containing protein	Antitoxin
sS8_RS09680	2246587..2247783	+	1197	WP_119629471.1	patatin-like phospholipase family protein	-
sS8_RS09685	2247871..2248422	+	552	WP_119629472.1	hypothetical protein	-
sS8_RS09690	2248969..2249142	+	174	WP_119629473.1	YqaE/Pmp3 family membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	pilU / pilT / gmd / clpV1 / hcp1 / hsiC1/vipB / hsiB1/vipA / gmd / htpB / cheW / cheY / flhA / tufA / ureG / ureA / sodB / tufA / htpB / flmH / acpXL / CBU_1566	535..6088915	6088380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-133)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (103-152)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17605.96 Da
• Isoelectric Point: 5.6829

>T31436 WP_119629469.1 NZ_AP017928:c2245897-2245424 [Methylocaldum marinum]
MRIWRISNYADLRGSGGIRAGGRWHSRGSPIVYCAENPGTALLEVLVHLEIDDPEDVPDSYQLLSIDLPDAVTRQELDRA
LLPDDWRGRSELTRSLGDTWLKNMSSAVLFVPSAIVPYTVNALINPLHSDSTRLRILAVDRYPFDERLFKGYGGTKP

Nucleotide

• Length: 474 bp

>T31436 NZ_AP017928:c2245897-2245424 [Methylocaldum marinum]
ATGCGCATCTGGCGCATCAGCAACTATGCGGATCTTCGTGGCAGCGGCGGCATTCGGGCCGGCGGACGCTGGCACAGCCG
TGGCAGCCCCATTGTCTATTGCGCCGAGAATCCGGGAACCGCTCTCCTCGAAGTGCTGGTGCATCTGGAGATCGATGATC
CTGAGGATGTGCCGGATAGTTATCAGTTGCTATCGATCGATCTACCGGATGCCGTGACCCGACAAGAACTCGACCGGGCA
TTGTTACCCGATGACTGGCGGGGGCGCTCGGAACTGACCCGATCACTGGGCGATACCTGGTTGAAGAATATGAGCAGTGC
TGTCCTGTTTGTGCCGAGCGCCATTGTCCCTTATACCGTTAATGCCCTTATTAACCCCCTCCATTCGGATAGCACTCGAC
TCCGGATTTTGGCCGTGGATCGGTACCCCTTCGATGAACGGCTTTTCAAAGGTTACGGCGGGACGAAACCTTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17463.12 Da
• Isoelectric Point: 8.5133

>AT31436 WP_119629470.1 NZ_AP017928:c2246364-2245897 [Methylocaldum marinum]
MVSRSNNTAAESSGSRYGHFLARLGILLDFPGRLTSEEELIREVPLRMQPSVLDKLAEEGLTAKELALVLPPRTLSHRRA
QQQPLSPDESDRALRVARLVALAETVFGNRDKALRWLRKPLKKLDYRTPMEILSTELGGRIIEEYLIQIDEGFAA

Nucleotide

• Length: 468 bp

>AT31436 NZ_AP017928:c2246364-2245897 [Methylocaldum marinum]
ATGGTATCCCGCTCCAACAACACCGCTGCCGAATCCAGTGGATCCCGCTACGGTCATTTTCTGGCACGTTTAGGTATCCT
GCTCGACTTTCCCGGACGCTTGACCTCGGAAGAGGAATTGATCCGCGAGGTACCGCTCCGGATGCAGCCGTCGGTCCTCG
ATAAGCTCGCCGAGGAGGGCTTGACGGCGAAAGAGCTGGCCTTGGTGCTTCCGCCCCGCACCCTTTCCCATCGGCGCGCC
CAGCAGCAACCCTTGTCGCCTGATGAGTCCGACCGGGCACTCCGCGTGGCTCGGCTCGTGGCCTTGGCGGAAACCGTCTT
CGGCAATCGGGACAAAGCCCTTCGCTGGCTACGCAAACCCCTCAAAAAGCTCGACTACCGAACGCCAATGGAGATCCTGT
CCACCGAACTGGGCGGGCGGATCATTGAAGAATACCTGATTCAAATCGATGAGGGCTTTGCGGCCTGA