Detailed information of TA system TA031435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 1890210..1890988
• Replicon: chromosome
• Accession: NZ_AP017928
• Organism: Methylocaldum marinum strain S8

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: sS8_RS08260
• Protein ID: WP_119629244.1
• Coordinates: 1890485..1890988 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: sS8_RS08255
• Protein ID: WP_119629243.1
• Coordinates: 1890210..1890488 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
sS8_RS08235	1885985..1887094	+	1110	WP_119629240.1	multicopper oxidase domain-containing protein	-
sS8_RS08240	1887256..1887726	+	471	WP_119629241.1	hypothetical protein	-
sS8_RS08250	1888706..1889683	+	978	WP_119629242.1	arsenic resistance protein	-
sS8_RS08255	1890210..1890488	+	279	WP_119629243.1	DUF1778 domain-containing protein	Antitoxin
sS8_RS08260	1890485..1890988	+	504	WP_119629244.1	GNAT family N-acetyltransferase	Toxin
sS8_RS08265	1891004..1891435	-	432	WP_119629245.1	LacI family transcriptional regulator	-
sS8_RS08270	1891432..1892786	-	1355	Protein_1625	recombinase family protein	-
sS8_RS08275	1892783..1892872	-	90	WP_197716711.1	hypothetical protein	-
sS8_RS28010	1892871..1893350	+	480	WP_197716712.1	hypothetical protein	-
sS8_RS08285	1893792..1894004	-	213	WP_119632666.1	helix-turn-helix domain-containing protein	-
sS8_RS08290	1894088..1895212	-	1125	WP_119629246.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1885985..1938925	52940
	-	inside	Integron	-	pilU / pilT / gmd / clpV1 / hcp1 / hsiC1/vipB / hsiB1/vipA / gmd / htpB / cheW / cheY / flhA / tufA / ureG / ureA / sodB / tufA / htpB / flmH / acpXL / CBU_1566	535..6088915	6088380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-88)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18359.14 Da
• Isoelectric Point: 7.2633

>T31435 WP_119629244.1 NZ_AP017928:1890485-1890988 [Methylocaldum marinum]
MSNDYSPVRKLAATDQVDAFDCGQAALNQFLQRYALVNQKANSAQTYVCCQGDVVVGFYSLAVGSVDPEAAPSRVMKGLA
RHPVPVMILARLAVDKEHQRKGLGQALLKDALLRTAQAADIAGIRCLLVHAKDDAARQWYESWEFEPSPTDPYHLLLMLK
DLKGMLS

Nucleotide

• Length: 504 bp

>T31435 NZ_AP017928:1890485-1890988 [Methylocaldum marinum]
TTGAGCAATGACTACTCACCCGTTCGCAAGCTCGCTGCTACGGATCAGGTCGATGCGTTCGACTGCGGCCAGGCCGCGCT
GAACCAGTTCCTGCAGCGCTACGCGCTCGTCAACCAGAAGGCCAACAGCGCGCAGACCTATGTCTGCTGCCAGGGTGACG
TGGTGGTCGGCTTCTACAGCCTGGCCGTTGGTAGCGTCGATCCGGAAGCCGCGCCGTCGAGAGTGATGAAGGGGTTGGCA
CGCCACCCGGTGCCGGTCATGATCCTGGCCCGGCTCGCCGTGGACAAGGAGCATCAGCGCAAGGGTCTGGGCCAGGCCTT
GCTCAAGGACGCCCTGCTGCGTACCGCACAGGCCGCCGACATCGCCGGCATTCGCTGCCTGTTGGTTCATGCCAAAGACG
ATGCCGCCCGTCAGTGGTACGAATCCTGGGAATTCGAGCCCAGCCCGACTGATCCGTACCATCTGCTCCTGATGCTCAAG
GATCTCAAAGGCATGTTGAGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10272.78 Da
• Isoelectric Point: 10.0988

>AT31435 WP_119629243.1 NZ_AP017928:1890210-1890488 [Methylocaldum marinum]
MSSLNLSKTERIDVRASTPVKQLLQEAARACHKNVSEFLLDAGVTAAAQTLADRRQFVLDDTQWQAFQEALDRPVQSKPR
LKKLLREPGVLG

Nucleotide

• Length: 279 bp

>AT31435 NZ_AP017928:1890210-1890488 [Methylocaldum marinum]
ATGAGCAGCCTGAATCTTTCCAAGACCGAGCGCATTGACGTTCGTGCCAGCACGCCGGTCAAACAACTGCTGCAGGAAGC
CGCACGCGCCTGCCACAAGAACGTCAGCGAGTTCCTGCTCGACGCGGGCGTGACGGCGGCTGCGCAGACTTTGGCGGATC
GTCGCCAGTTCGTGCTGGACGACACGCAGTGGCAGGCCTTCCAGGAAGCGCTAGATCGGCCGGTGCAGAGCAAGCCGCGT
CTGAAGAAGTTGCTGCGTGAACCTGGGGTGCTGGGTTGA